Ty-Jour T1 - 在Atopic Athma JF中的支气管粘膜中mRNA和MCP-3的表达增加 - 欧洲呼吸期Jo - Eur Respir J SP - 2454 LP - 2460 VL - 9是 - 12 Au - Powell，N Au- Humbert，M Au - Durham，SR Au - Assoufi，B Au - Kay，AB Au - Corrigan，CJ Y1 - 1996/12/01 UR - //www.qdcxjkg.com/content/9/12/2454.Abstract N2 - 嗜酸性粒细胞的选择性募集到气道的粘膜衬里是特应哮喘的突出特征，并且被认为是疾病发病机制中的重要组成部分。负责嗜酸性粒细胞涌入的精确刺激仍不清楚。使用半定量逆转录酶聚合酶链反应（RT-PCR）技术，拷贝的数量（相对于编码嗜酸性粒细胞活性趋化性细胞因子的信使核糖核酸（mRNA）的拷贝的数量，该因子受到调节的因子在来自Atopic哮喘患者（n = 9）的支气管活组织检查中，测量正常T细胞中的常规T细胞和单核细胞趋化蛋白-3（MCP-3），与特应性无发血（n = 8）和非储物非发育（n = 8）对照受试者。此外，为来自每个受试者的进一步的活组织检查用于免疫组织化学和测量的活化（EG2 +）嗜酸性粒细胞的数量。与特方向性无发血对照（P = 0.013）和非饮用性非发育对照（P = 0.007）相比，rantes mRNA的表达明显升高。类似地，相对于特应性非惊厥对照（P = 0.014）和非饮品非发育对照组（P = 0.011），在特应性哮喘基团中，MRNA编码MCP-3的表达明显升高。 Elevated RANTES and MCP-3 mRNA expression was associated with significantly increased numbers of bronchial mucosal eosinophils in the atopic asthmatic patients as compared to the atopic nonasthmatic (p = 0.03) and nonatopic nonasthmatic (p = 0.006) control subjects. In conclusion, we have identified elevated expression of messenger ribonucleic acid encoding RANTES and monocyte chemotactic protein-3 in the bronchial mucosa of atopic asthmatic patients relative to controls. These findings are compatible with the hypothesis that eosinophil-active beta-chemokines play a role in the mechanism of eosinophil recruitment to the asthmatic bronchial mucosa. ER -