TY -的T1 -人类血液单核细胞,但不是肺泡巨噬细胞,揭示增加CD11b / CD18表达式和粘附性能在receptor-dependent激活JF -欧洲呼吸杂志》乔和J SP - 1188 LP - 1194欧元六世- 9 - 6盟Lundahl J盟——Hallden G AU - Skold教授,2整合素受体CD11b/CD18介导内皮内层以及细胞外基质成分的粘附。本研究旨在研究外周血单核细胞(BMs)和肺泡巨噬细胞(AMs)中CD11b/CD18的定量水平以及与激活状态相关的黏附特性。从健康受试者中招募脑转移体和脑转移体。采用流式细胞术定量分析CD11b/CD18的表面表达，并对静息和n-甲氧基-亮基-苯丙氨酸(fMLP)激活的细胞进行白蛋白包被表面的粘附特性。受体非依赖性刺激(phorboll -12- myriate -13-acetate (PMA)和离子霉素)被用于附加实验。流式细胞术和免疫荧光显微镜检测细胞内储存的CD11b/CD18。CD11b/CD18在静息脑转移瘤表面表达增加5倍(p <0.01)。在静息状态下，CD11b/CD18的表面表达显著升高(p < 0.01) compared to resting BMs but did not increase further upon activation with fMLP, PMA or ionomycin. In contrast to BMs, no evidence for an additional intracellular pool of CD11b/CD18 was found in AMs. The adherence of resting BMs did not significantly differ from the adherence of resting AMs. After fMLP activation, the adherence of BMs, but not AMs, increased significantly (p < 0.05). Our results indicate that in vivo differentiation of human blood monocytes into alveolar macrophages implies reduced responsiveness to fMLP in terms of CD11b/CD18 upregulation and adhesion properties, and that the lack of upregulation of CD11b/CD18 on alveolar macrophages presumably depends on the absence of an additional intracellular pool. ER -