RT期刊文章SR电子T1测量诱导痰液中炎症指数的测量：选择痰液的效果以最大程度地减少唾液污染JF欧洲呼吸杂志Jo Eur Respir J FD欧洲呼吸学会SP 1174 OP 1180 vo 9 IS 6 A1 Pizzichini，e a1 pizzichini，e a1 pizzichini，e a1 pizzichini，e a1 pizzichini，e a1 pizzichini，e a1 pizzi188bet官网地址chini，e a1 pizzichiniMM A1 efthimiadis，A1 Hargreave，Fe A1 Dolovich，J YR 1996 UL //www.qdcxjkg.com/content/9/6/1174.Abstract ab Abstract Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Ab Abs Trupum检查正在越来越多地评估Airway炎症的方法。预期的痰液对唾液具有可变的污染。加工方法包括选择样品的选择，被认为是肺部来源与使用整个样品的使用，这是由不同体积的唾液混淆的。我们比较了从期望酸盐和通常丢弃的残留部分中选择的痰液中细胞谱和嗜酸性阳离子阳离子蛋白（ECP）浓度，以确定唾液污染在多大程度上最小化，并且结果是否代表了下呼吸道分泌。在六名健康和九个哮喘患者中，用高渗盐水诱导痰液。从残留物中选择了所有被认为是纯下呼吸道起源的部分。用二硫代硫醇，总细胞计数和细胞活力处理所选的和残留部分，并为差分细胞计数进行细胞固定，并收集上清液以进行ECP测定。与残留部分相比，选定的样品部分显示：鳞状细胞污染很少（中位1.2 vs 70％； p <0.001）；ML-1（5.1 vs 0.5 x 10（6）单元格。 higher number of viable nonsquamous cells per sample (1.9 vs 0.6 x 10(6) cells; p < 0.001); higher slide quality score (7 vs 4; p < 0.001); and higher levels of ECP (768 vs 136 micrograms.L-1; p < 0.001). There were no differences in the differential cell counts of eosinophils (1.3 vs 3.8%), neutrophils (44 vs 32%), and lymphocytes (0.6 vs 0.6%). While the proportion of macrophages was lower (36 vs 54%; p < 0.05), the absolute number (41 vs 19 x 10(4) cells; p < 0.05) was higher in the selected portion. In summary, selection of all portions of induced sputum from the expectorate minimized the confounding influence of saliva. Loss of nonsquamous cells in the residual portion was variable but usually less than one third of those in the selected portion. With one exception, this loss had little influence on the differential counts of inflammatory cells. Similar observations apply to eosinophilic cationic protein levels. We conclude that, in healthy subjects and treated asthmatics, inflammatory markers in the selected portion of the expectorate can be used to represent those in the lower respiratory tract in general.