TY -的T1 -氧化BAL患者液体蛋白质间质性肺疾病JF -欧洲呼吸杂志》乔和J SP - 307 LP - 312欧元六世- 9 - 2非盟楞次AG盟——Costabel U AU -迈尔,KL Y1 - 1996/02/01 UR - //www.qdcxjkg.com/content/9/2/307.abstract N2 - Oxygen-derived自由基,发布的吞噬细胞,被假定为肺组织损伤。因此,我们研究了氧化损伤蛋白从支气管肺泡灌洗液(BALF)作为氧化应激的指标和评估在肺部抗氧化防御系统。我们检查了BAL液体从间质性肺疾病患者,如特发性肺纤维化(IPF),不吸烟者(NS)和吸烟者(S)),结节病(不仅不吸烟者),石棉肺(ASB,抽过烟(练习)。BALF蛋白质的氧化是伴随着羰基化合物的引入到他们的氨基酸侧链可以量化和标签与硼氢化氚化这些团体。氧化蛋白质的肺总内容恢复了支气管肺泡灌洗(BAL)是0.3 + / - 0.07 nmol羰基。mL-1 BALF(意思是+ / - SEM)在NS组(n = 9)倾向于增加,在无症状组(n = 8;0.59 + / - 0.14 nmol.mL-1)。这个参数显著升高在IPF-NS (n = 14;0.84 + / - 0.2 nmol羰基。mL-1 BALF)和SARC-NS (n = 15; 0.73 +/- 0.16 nmol.mL-1) as compared with the NS control. On the contrary, in smoking patients with IPF (n = 6; 0.41 +/- 0.1 nmol carbonyl.mL-1 BALF) and also in ASB-EXS (n = 6; 0.37 +/- 0.06 nmol.mL-1) it was not different from NS controls. The total amount of oxidized proteins correlated positively with the absolute number of eosinophils (EOS) in IPF-NS, IPF-S and SARC, and also with absolute polymorphonuclear neutrophil (PMN) numbers in IPF-NS and IPF-S. In conclusion, oxidative damage of BALF proteins occurred in nonsmoking patients with IPF and SARC. The amount of oxidized bronchoalveolar lavage fluid protein may provide a quantitative assessment of oxygen burden, a balance between oxidant stress and antioxidant defences. ER -