Ty-jour t1 - 牛气管反应性在体外：上皮和一氧化氮的作用JF - 欧洲呼吸杂志Jo - Eur Respir J SP - 2286 LP - 2293 VL - 9是 - 11 Au - Sadeghi-Hashjin，G Au - Henricks，Pa Au - Folkerts，G Au - Verheyen，AK Au - Van der Linde，HJ Au - Nijkamp，FP Y1 - 1996/11/01 UR - //www.qdcxjkg.com/content/9/11/2286。抽象N2 - 气道上皮释放抑制因子，例如一氧化氮（NO）和前列腺素E2（PGE2），其可以抵消支气管机会。我们研究了上皮衍生的抑制物质是否对体外对牛气管反应性发挥至关重要的影响。比较了响应于完整和上皮脱落的气管平滑肌条的组胺的等渗和等距收缩。此外，L-精氨酸（L-ARG），N（g） - 尼硝基-1-精氨酸甲基亚斯（L-名称）和N（g） - mon甲基L-精氨酸（L-NMMA）对组胺的影响调查了响应能力。测量来自气管上皮的NO和PGE2的释放。从气管平滑肌条除去上皮的去皮并未改变产生半胺的浓度的阴性日志，其产生半效应（PD2）或最大效果（Emax）。用L-ARG或L-NAME孵育组织25分钟，没有影响组胺的基础音调或诱导的收缩。然而，与L-NMMA孵育增加基础音调并导致组胺的轻微低位。 S-nitroso-N-acetyl-penicillamine (SNAP, a direct NO donor) reversed the contraction induced by histamine in a concentration-dependent manner. Stimulation of the epithelial layer by 0.1 microM histamine increased the release of NO 3-4 fold compared to basal levels; this effect was completely inhibited in the presence of L-NMMA. In addition, 1 mM histamine caused a significant increase in the release of PGE2 from the epithelial tissue. In conclusion, no functional inhibitory influence of the epithelium can be identified in bovine airways. The S-nitroso-N-acetyl-penicillamine-induced relaxation demonstrates the presence of a nitric oxide sensitive pathway in bovine airways. However, the amounts of nitric oxide and prostaglandin E2 released from bovine tracheal epithelium are probably too low to exert a significant effect on the histamine-induced contractions. ER -