％0期刊文章valdes，l％san jose，e％a alvarez，d％a valle，jm％t腺苷脱氨酶（Ada）胸膜沸腾中的同工酶分析：诊断作用，与增加的Ada的起源相关结核性葡萄牙语％D 1996％J欧洲呼吸轴颈％P 747-751％p 747-751％v 9％n 4％x腺苷脱核酶（Ada）活性在结核性胸膜患者的胸膜液中的上升，虽然用于诊断，属于未知的起源。在这项工作中，我们确定了350名患者中2'-脱氧腺苷脱氨酶和ADA-2的活动和ADA-2的活动。我们还考虑了结果在结核性胸膜炎中的高胸膜液体ADA的起源并估计了2'-脱氧腺苷脱氨酶，ADA-2和总ADA活性的诊断效率，并不包含了2'-脱氧腺苷脱氨基酶/ ADA活动比例在组合标准中。通过先前建立的标准作为翻译（60名男性/ 18名女性）或结核（49名男性/ 27名女性），肿瘤（50名男性/ 39名女性），肺炎（36名男性/ 19名女性），脓血（36名男性/ 19名女性），脓包（11个男性/ 3名女性），或杂项（25名男性/ 13名女性）渗出。分别为127.5 +/- 2.9,103 +/- 2.9,103 +/-29.5和42.8 +/-14 U.L-1分别为The Ada，Ada-2和2'-脱氧腺苷脱氧酶活性。分别具有47,40和22 u.L-1的诊断阈值，ADA，ADA-2和2'-脱氧腺苷脱氧糖酶用于结核病的敏感性为100,100和95％;他们的特异性91,96和92％;他们的效率分别为93,97和93％。一百个潮流（全部76个结核，12个肿瘤，4例，4例脓疱剂）具有全ADA水平> 47 U.L-1; of these, 8 neoplastic, 1 parapneumonic and all the tuberculous exudates had a 2'-deoxyadenosine deaminase/ADA activity ratio < 0.49. The criterion of simultaneously having ADA > 47 U.L-1, ADA-2 > 40 U.L-1 and a 2'-deoxyadenosine deaminase/ADA activity ratio < 0.49 was satisfied by all the tuberculous effusions but only eight others (all neoplastic) (sensitivity 100%, specificity 97%, efficiency 98%). We conclude that: 1) high total ADA activity in tuberculous pleural effusions is due mainly to an increase in ADA-2, and, therefore, originated from the only known source monocytes and macrophages; 2) ADA-2 was a more efficient diagnostic marker of tuberculous pleurisy than total ADA activity, although the difference was not statistically significant; and 3) among effusions with high total ADA the 2'-deoxyadenosine deaminase/ADA activity ratio differentiates tuberculous effusions from empyemas and parapneumonic effusions, but fails to discriminate well between tuberculous and neoplastic effusions. %U //www.qdcxjkg.com/content/erj/9/4/747.full.pdf