TY - T1的不同的功能和形态特征的不依从族群人类巨噬细胞恢复了支气管肺泡灌洗JF -欧洲呼吸杂志》乔和J SP - 1719 LP - 1724欧元六世- 8 - 10盟Skold教授厘米盟——Barck C盟——Lundahl J AU -约翰逊,Y1 - 1995/10/01 UR - //www.qdcxjkg.com/content/8/10/1719.abstract N2肺泡巨噬细胞(AMs)构成形态学和功能异质的细胞群。这些细胞的粘附特性对他们在宿主防御的角色很重要。专注于异质性,人口的不依从巨噬细胞在功能和形态特征。这些细胞被肺泡巨噬细胞总数相比。Alveolar cells (> 95% alveolar macrophages), were recovered by bronchoalveolar lavage (BAL) from healthy smokers. Nonadherent macrophages were separated by adhesion. The phagocytic capacity and the autofluorescent properties of the cell populations were determined in flow cytofluorometric assays. In addition, electron microscopic evaluation was performed. The alveolar macrophage adhesion to wells coated with albumin increased in a time-dependent manner. After 15 min, median 47%, interquartile range 42-52% (uncoated wells 68%, 67-72%) of the alveolar macrophages were attached; and after 60 min, 56%, 51-58% (uncoated wells 73%, 71-76%) of the alveolar macrophages were attached. The nonadherent alveolar macrophage population had less phagocytic capacity. The cell autofluorescence increased with increasing cell size and cell complexity/granularity in both populations. The nonadherent cells were more autofluorescent, indicating an increased granularity/complexity. These findings were confirmed with electron microscopy. Thus, the nonadherent alveolar macrophages had more cytoplasmic inclusions than the total alveolar macrophage population (volume density median 0.39, interquartile range 0.35-0.46 and 0.31, 0.26-0.34, respectively), but less surface protusions. We conclude that in lavage fluid from human smokers there is an ultrastructurally specific subpopulation of alveolar macrophages, showing less adhesive properties and impaired phagocytic capacity in vitro. These macrophages may be older alveolar cells or, alternatively, airway macrophages. Since only alveolar macrophages from smokers were investigated, we cannot draw any conclusions regarding alveolar cells from nonsmokers. Nevertheless, the heterogeneity of the lavaged cells should be taken into consideration when the functional ability of the alveolar macrophages are evaluated. ER -