TY -的T1 -急性吸烟对肺表面活性物质的分泌的影响大鼠肺泡II型细胞在文化摩根富林明——欧洲呼吸杂志乔-欧元和J SP - 24 LP - 32六世- 9 - 1 AU Wirtz人力资源非盟-施密特,M Y1 - 1996/01/01 UR - //www.qdcxjkg.com/content/9/1/24.abstract N2 -它已经证明,吸烟会导致较低的表面活性剂产量相关磷脂在支气管肺泡灌洗(BAL)。间接证据表明分泌功能受损。在本研究中，我们研究了香烟烟雾对培养的大鼠肺泡II型细胞表面活性物质分泌的影响。烟雾暴露是通过将四根香烟的烟雾通过杜尔贝克改良的Eagle's medium (DMEM)进行冒泡来实现的，该介质在320 nm处的参考吸收值调整为1.36。细胞在不同浓度的香烟处理培养基中孵育30分钟，然后暴露于该培养基2小时。之后，测量3h -胆碱标记的磷脂酰胆碱(PC)的分泌，作为表面活性剂分泌的标记。将香烟处理过的培养基稀释10倍后，特布他林、三磷酸腺苷和12- o -十四烷基福尔博-13-乙酸联合使用可抑制PC分泌50%以上，但不改变基础分泌。暴露于浓度较低的香烟处理过的介质导致较少的抑制。在本研究中，使用香烟处理的培养基浓度未观察到细胞损伤。香烟烟雾的气相在相对浓度下没有抑制作用。较长时间暴露于香烟处理过的介质中，可增加对PC分泌的抑制。 The cigarette smoke ingredients, nicotine and benzo[a]pyrene, failed to inhibit PC secretion. Secretion of type II cells exposed to cigarette smoke-treated medium at lower temperatures was not affected. Addition of antioxidants to medium and cells during the preincubation and secretion period did not alter cigarette smoke-treated medium-induced inhibition of stimulated PC secretion. These results demonstrate a direct inhibitory effect of cigarette smoke constituents on surfactant secretion in type II cells. Inhibition is mediated by compounds contained predominantly in the particulate phase of cigarette smoke. Inactivation of the inhibitory effect by lower temperatures suggests involvement of processes such as enzymatic bioactivation or active transport mechanisms. ER -