TY - JOUR T1 - 对肺表面活性物质分泌在培养JF香烟烟雾的急性影响在大鼠肺泡II型细胞 - 欧洲呼吸杂志JO - EUR呼吸杂志SP - 24 LP - 32 VL - 9 - 1 AU - 维尔茨，HRAU - 施密特，男Y1 - 1996年1月1日UR - //www.qdcxjkg.com/content/9/1/24.abstract N2 - 它已被证明的是，在表面活性剂的产率较低吸烟结果相关联磷脂支气管肺泡灌洗液（BAL）。间接证据表明分泌受损。在本研究中，我们在培养的大鼠肺泡II型细胞研究了表面活性物质分泌的香烟烟雾的影响。烟雾暴露是由通过Dulbecco改良的Eagle培养基（DMEM），其在320nm处调节到1.36的参考吸收值鼓泡四个香烟烟雾实现。将细胞用30分钟香烟烟雾处理的介质的各种稀释液预温育，并随后暴露于该培养基中保持2小时。此时间之后，3H胆碱 - 标记的磷脂酰胆碱（PC）的分泌被测量为表面活性物质分泌的标记。香烟烟雾处理介质抑制PC分泌的10倍稀释液刺激由特布他林，三磷酸腺苷和佛波醇-12-十四烷酰-13-乙酸酯的组合，通过在50％以上，但不改变基础分泌。暴露于浓度较低的香烟烟雾处理的介质导致抑制少。 Cellular injury was not observed with the concentrations of cigarette smoke-treated medium used in this study. The gas phase of cigarette smoke was not inhibitory at comparable concentrations. Longer exposure to cigarette smoke-treated medium resulted in increased inhibition of PC secretion. The cigarette smoke ingredients, nicotine and benzo[a]pyrene, failed to inhibit PC secretion. Secretion of type II cells exposed to cigarette smoke-treated medium at lower temperatures was not affected. Addition of antioxidants to medium and cells during the preincubation and secretion period did not alter cigarette smoke-treated medium-induced inhibition of stimulated PC secretion. These results demonstrate a direct inhibitory effect of cigarette smoke constituents on surfactant secretion in type II cells. Inhibition is mediated by compounds contained predominantly in the particulate phase of cigarette smoke. Inactivation of the inhibitory effect by lower temperatures suggests involvement of processes such as enzymatic bioactivation or active transport mechanisms. ER -