TY-JUR T1 - 嗜血杆菌流感内毒素对IL-6，IL-8，TNF-α和ICAM-1表达的合成的影响JF - 欧洲呼吸期Jo - Eur Respir J SP - 2109LP - 2116 VL - 7是 - 12 AU - Khair，OA Au - Devalia，JL Au - Abdelaziz，MM Au - Sapsford，RJ Au - Tarraf，H Au - Davies，RJ Y1 - 1994/12/01 UR - HTTP：//www.qdcxjkg.com/content/7/12/2109.Abstract n2 - 尽管感染性肺病的研究表明，嗜血杆菌嗜血杆菌是一个主要病原体，这种生物体的发病机制尚不清楚。我们将人支气管上皮细胞（HBEC）培养至汇合，并研究了HBEC中14叔牛血清白蛋白（14C-BSA）的上皮渗透性的嗜型流感内毒素（HIE）的疗效和测量HBEC的电阻;2）通过酶联免疫吸附试验（ELISA）释放白细胞介素-6（IL-6），白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）;3）通过免疫荧光染色的细胞间粘附分子-1（ICAM-1）的表达。HIE没有显着增加HBEC跨越14C-BSA的运动。相比之下，HIE逐渐增加了HBEC的电阻，使得24小时后显着显着。与未处理的细胞相比，10-100微克.ML-1 HIE处理的细胞在24小时后释放出大量的IL-6，IL-8和TNF-α，其被10（-5）米的氢化胞蔻体封闭。 Similarly, incubation of HBEC with 10-100 micrograms.ml-1 HIE, significantly increased the total number of ICAM-1 positive cells, which were significantly decreased on incubation of the cells in the presence 10(-5) M hydrocortisone. Conditioned medium from HIE-exposed HBEC lead to significant increase in neutrophil chemotaxis and adhesion to endothelial cells in vitro. These results suggest that HIE may affect epithelial cell function and influence inflammation of the airway mucosa via induction of proinflammatory mediators. ER -