TY-JUR T1 - 血小板激活因子诱导的超氧化物阴离子释放因吸烟者的人肺泡巨噬细胞增加JF - 欧洲呼吸期刊JO - EUR RESPIR J SP - 387 LP - 393 VL - 5是 - 4 Au - Schaberg，T Au- Haller，H Au - Rau，M Au - Kaiser，D Au - Fassbender，M Au - Lode，H Y1 - 1992/04/01 UR - //www.qdcxjkg.com/content/5/4/387。abstract N2 - This study was designed to investigate the effects of the platelet-activating factor (PAF) on the superoxide anion production (O2.) of human alveolar macrophages (AM) from nonsmoking (n = 18) and smoking (n = 30) subjects. Freshly isolated cells were stimulated with (PAF) or with a phorbol ester (phorbol 12-myristate 13 acetate (PMA)). Stimulation with PAF led to a dose-dependent increase of O2. production by AM in both groups. The median effective dose (EC50) for PAF action on O2. production of smoker AM was 0.5 x 10(-8) M, compared to nonsmoker AM with an EC50 of 1.0 x 10(-7) M. This effect of PAF was blockable by the PAF-antagonist WEB 2086 in a dose-dependent manner. Comparison of the relative increase of O2. production after PAF-stimulation showed that smoker cells were significantly more sensitive to PAF than nonsmoker cells (p less than 0.01). In contrast to the findings with PAF, the relative increase of O2. production after PMA-stimulation showed no differences between smoker and nonsmoker AM. Our data suggest that AM from smoking subjects are more sensitive to PAF than AM from nonsmokers. ER -