PT-日记文章Au -Pons，A。R. Au -Sauleda，J。Au -Noguera，A.Au -Pons，J。Au -Barceló，B。Au -Au -Fuster，A.Au -Auustí，A。G. N. Ti-减少了巨噬细胞的释放慢性阻塞性肺疾病帮助中的TGF -β和TIMP -1 -10.1183/09031936.05.00045504 DP- 2005年7月01日-TA-欧洲呼吸杂志PG -60--66 VI -26 IP -1 4099 -HTTP：//ERJ.ERSJOURNALS。26 AB-本研究检验了以下假设：慢性阻塞性肺疾病（COPD）患者的肺泡巨噬细胞（AM）释放比具有正常肺功能和从不吸烟者的吸烟者的抗炎介质更多的促炎和/或抗炎介质更多。AM通过13例COPD患者的支气管肺泡灌洗液的流式细胞仪分类（平均值±SEM 67±2岁，一秒钟（FEV1）61±4％参考），16名吸烟者，具有正常肺功能（55±2） yrs, FEV1 97±4% reference) and seven never-smokers (67±7 yrs, FEV1 94±4% reference). After sorting, AM were cultured (with and without lipopolysaccharide stimulation) after 4 h and 24 h, and the concentrations of leukotriene B4 (LTB4), transforming growth factor (TGF)-β1 and tissue inhibitor of metalloproteinase (TIMP)-1 were quantified in the supernatant by ELISA. The production of reactive oxygen intermediates (ROI) in freshly isolated AM was determined by flow cytometry. LTB4 secretion and ROI production were not different between groups. In contrast, AM from COPD patients released significantly less TGF-β1 and TIMP-1 than those from smokers with normal lung function and nonsmokers. In conclusion, these observations are compatible with reduced anti-inflammatory and anti-elastolytic capacity in chronic obstructive pulmonary disease, which is likely to contribute to the pathogenesis of the disease.