TY -的T1 - CC和C趋化因子表达在肺结节病JF -欧洲呼吸杂志》乔-欧元和J SP - 1206 LP - 1212 - 10.1183 / 09031936.02.00289902六世- 20 - 5盟Petrek m . AU - Kolek诉盟——Szotkowska J . AU -杜波依斯R.M. Y1 - 2002/11/01 UR - //www.qdcxjkg.com/content/20/5/1206.abstract N2 -趋化因子RANTES(激活、t细胞表达和分泌调节;CC趋化因子配体(CCL)-5)和单核细胞炎症蛋白(MIP)-1α (CCL-3)与肺结节病的肺泡炎的发展有关。新的C趋化因子单半胱氨酸基元(SCM)-1α (XCL-1)和CC趋化因子单核细胞趋化蛋白(MCP)-1 (CCL-2)也是单核细胞趋化因子，代表肺泡炎的备选候选介质。因此，我们在对照组和结节病患者的支气管肺泡灌洗液(BALF)中研究MCP-1和SCM-1α的表达，以及RANTES和MIP-1α的表达。并探讨趋化因子表达与结节病临床病程的关系。采用半定量逆转录-聚合酶链反应从未分离的支气管肺泡细胞中提取RNA(17例患者，12例对照组)，测定所有4种趋化因子的信使核糖核酸(mRNA)表达。采用非浓缩BALF酶联免疫吸附法测定RANTES、MIP-1α和MCP-1蛋白(60例患者，17例对照组)。MCP-1，即RANTES和SCM-1α mRNA表达在结节病中上调，特别是在病情较晚期的患者中。RANTES，即患者BALF样本中MCP-1浓度升高; MCP-1 levels were most increased in patients with chest radiographic stage 2 disease and also in patients with persistent and recurrent disease. In conclusion, chemokines monocyte chemotactive protein-1 and single cysteine motif-1α are, in addition to RANTES, associated with the development of alveolitis in sarcoidosis and their expression parallels the disease course. This study was supported by the Czech Ministries of Health (IGA 3768-3) and of Schools, Education and Physical Training (MSM 151100002). ER -