TY - T1的微卫星DNA不稳定性和杂合性丢失在支气管哮喘JF -欧洲呼吸杂志》乔欧元和J SP - 951 LP - 955 - 10.1183 / 09031936.03.00010503六世- 22 - 6盟Paraskakis e . AU - Sourvinos g . AU - Passam f . AU - Tzanakis联合国非盟- Tzortzaki,如非盟- Zervou m . AU - Spandidos d . AU - Siafakas,新墨西哥州Y1 - 2003/12/01 UR - //www.qdcxjkg.com/content/22/6/951.abstract N2 -基因改变,如杂合性缺失(LOH)或微卫星不稳定性(MI)，在恶性和良性疾病中都有报道。为确定哮喘患者脱氧核糖核酸(DNA)突变位点，对痰细胞进行了心肌梗死(MI)和LOH的研究。从22名中度哮喘患者的痰细胞和血细胞中提取DNA。利用染色体5q, 6p, 11q, 14q上的18个多态性标记分析细胞MI和LOH。还对6名健康受试者进行了微卫星分析。没有一个健康的个体表现出任何基因改变。22例哮喘患者中有16例(73%)发现了基因改变。共有12例(54.5%)患者仅表现为LOH, 1例(4.5%)仅表现为MI, 3例同时表现为MI和LOH。染色体14q上LOH和MI的发生率最高。 Mean immunoglobulin E and blood eosinophil levels were significantly higher in asthmatics with three or more genetic alterations. A high incidence of genetic alterations in the deoxyribonucleic acid of the sputum cells was found in asthmatic patients. Further studies are needed to identify the role of loss of heterozygosity and microsatellite instability in the investigation of genetic susceptibility of asthma and thus, in its pathogenesis. ER -