Ty -jour t1-具有重大组织相容性复合物II类和共刺激分子的外泌体中存在于人类BAL流体JF-欧洲呼吸杂志JO -EUR RESSIR J SP -578 LP -578 LP -583 DO -10.1183/09031936.09031936.03.0003.0003.00033.00041703 VL -22-4 au -admyre，C.Au -Grunewald，J.Au -Thyberg，J.Au -Gripenbäck，S。Au -au -tornling，G.Au -eklund，A.Au -au -Scheynius，A.Au -Gabrielsson，S.S.S.S.S.Y1-2003/10/01 UR -http：//www.qdcxjkg.com/content/22/4/578.abstract N2-外泌体是30-100 nm直径的囊泡，由内体隔板的内向萌芽形成，并由内体隔板产生，并且由细胞类型，包括T细胞，B细胞和树突状细胞（DC）。DC的外泌体表达主要的组织相容性复合物（MHC）I和II类，以及其表面的共刺激分子，并可以诱导T细胞的抗原特异性激活。本研究的目的是调查健康个体的支气管肺泡灌洗液（BALF）中的外泌体，并确定这些外泌体是否含有MHC和共刺激分子。作者分析了从七名健康志愿者那里获得的BALF，并从单核细胞来源的DC（MDDC）培养物中使用了外泌体作为参考。超速离心后，外泌体与抗MHC II类涂层磁珠结合，并通过流式细胞仪和电子显微镜进行分析。作者首次报告说外泌体存在于BALF中。这些外泌体类似于MDDC衍生的外泌体，因为它们表达了MHC I和II，CD54，CD63和共刺激分子CD86。 The results demonstrate that exosomes are present in the lung, and since they contain both major histocompatibility complex and co-stimulatory molecules it is likely that they are derived from antigen presenting cells and might have a regulatory role in local immune defence. This work was supported by the Swedish Research Council (grants no. 16x-7924, 74x-14182, and 6537), the Swedish Council for Work Life Sciences, the Swedish Asthma and Allergy Association's Research Foundation, the Hesselman Foundation, Konsul TH Bergs Foundation, the Swedish Heart‐Lung Foundation, the Vårdal Foundation, the Centre for Allergy Research at Karolinska Institutet, the King Oscar II Jubilee Foundation, the King Gustaf V 80th Birthday Fund and the Karolinska Institutet. ER -