TY - T1的取向和上皮细胞的存在是关键endotoxin-induced中性粒细胞迁移JF -欧洲呼吸杂志》乔和J SP - 1053 LP - 1059欧元六世- 11 - 5 AU -佩拉尔塔,FM AU -萨莱,结核病Y1 - 1998/05/01 UR - //www.qdcxjkg.com/content/11/5/1053.abstract N2 -木糖醇的机制调节中性粒细胞transepithelial迁移和肺部炎症尚不清楚。这是假设的存在和取向上皮细胞中性粒细胞迁移的endotoxin-induced至关重要。中性粒细胞迁移是通过赤裸的过滤器和过滤器相比A549肺上皮层上生长的上、下表面渗透过滤器来模拟中性粒细胞的顶端和基底定向运动,分别。内毒素,铜绿假单胞菌脂多糖,被以下过滤器,作为基底或一个顶端的刺激。没有血清内毒素未能刺激中性粒细胞迁移。在1%的人类血清,endotoxin-induced中性粒细胞迁移通过裸体过滤器是剂量依赖性。Endotoxin-induced中性粒细胞迁移在A549单层膜层时最小是培养的上表面过滤器(基底刺激)。相比之下,中性粒细胞transepithelial迁移更大,单层时依赖于剂量和时间培养的下表面过滤器(基底顶端中性粒细胞定向运动)。此外,增强中性粒细胞transepithelial迁移与基底的顶端大于刺激。Endotoxin-induced neutrophil transepithelial migration was markedly inhibited (>95%) by actinomycin D pretreatment of the monolayers, suggesting the necessity for intact protein synthesis capacity of the A549 cells. Thus, both the presence and orientation of airway epithelium are key in supporting endotoxin-mediated lung neutrophilic responses. ER -