PT -期刊文章盟Kuwano K野本盟- Y盟——Kunitake R盟——Hagimoto N盟——Matsuba T非盟-录像,Y盟——Hara N TI -检测腺病毒E1A基因在肺纤维化使用嵌套式聚合酶链反应DP - 1997年7月01 TA -欧洲呼吸杂志》第六PG - 1445 - 1449 - 10的IP - 7 4099 - //www.qdcxjkg.com/content/10/7/1445.short 4100 - //www.qdcxjkg.com/content/10/7/1445.full所以欧元和J1997 7月01;10 AB -患者的历史特发性肺纤维化(IPF)表明,该疾病可能之前viral-like疾病。虽然病毒没有了,可能是没有检测到病毒在文化因为他们不复制延迟。我们在IPF研究腺病毒的存在,与胶原血管疾病相关的间质性肺炎(CVD-IP),使用嵌套式聚合酶链反应(PCR)和原位杂交(ISH) E1A的腺病毒基因组区域。transbronchial获得的研究进行肺组织肺活检从19 IPF患者,10例CVD-IP,相比之下,来自20个结节病。E1A的DNA存在于3 19(16%)例IPF, 5 10(50%)例CVD-IP, 20(10%), 2例结节病。The incidence of E1A DNA in CVD-IP was significantly higher than that in sarcoidosis (p<0.05). In patients with IPF and CVD-IP, E1A DNA was more prevalent in patients treated with corticosteroids (6 out of 9 cases; 67%) than in those without it (2 out of 20 cases; 10%) (p<0.01). ISH studies showed that 1 out of 8 cases of IPF and CVD-IP, in which E1A DNA was detected by PCR, was positive for E1A DNA. We conclude that adenovirus E1A is unlikely to be aetiologically involved in the pathogenesis of idiopathic pulmonary fibrosis or interstitial pneumonia associated with collagen vascular disease. However, a latent adenovirus infection may be reactivated or may newly infect the host following corticosteroid administration.