Boitelle % 0期刊文章%,%戈塞特,P % Copin, MC % Vanhee, D %马奎特,CH % Wallaert, B % Gosselin, B % Tonnel, AB % T MCP-1分泌的肺不吸烟患者煤炭工人肺尘埃沉着病% D J欧洲呼吸杂志1997% % P 557 - 562 X % V 10% N 3%接触煤尘的发展导致煤炭工人肺尘埃沉着病(CWP),疾病与巨噬细胞的积累下呼吸道。招聘机制控制单核细胞仍知之甚少。由于单核细胞化学引诱物蛋白1 (MCP-1)被认为是一个强有力的血液单核细胞的趋化现象的因素,我们分析了存在MCP-1 CWP患者的肺隔间。支气管肺泡灌洗液(BALF)从16不吸烟对照组27不吸烟的CWP患者(16使用简单的肺尘埃沉着病(SP)和11与进步的巨大纤维化(及)进行了分析。肺泡巨噬细胞(AMs)纯化的依从性和BALF被冻干集中十倍。MCP-1测量BALF和3 h是上层清液使用夹心酶联免疫吸附试验(ELISA)。MCP-1本地化的肺组织是由免疫组织化学组织部分从三个CWP患者和两个对照组。MCP-1水平明显高于集中BALF中SP或及患者(分别为平均370 - 555 pg x mL-1)比那些来自对照组(平均11 pg x mL-1) (p < 0.001)。MCP-1公布在我浮在表面的增强CWP患者(平均83 pg x mL-1),但相比控制(平均41 pg x mL-1)这个级别没有达到意义。虽然显著增加,是计数CWP患者BALF中没有与MCP-1水平。 MCP-1 levels in BALF correlated with MCP-1 levels in AM supernatants (p=0.47; p<0.02). In control lung specimens, MCP-1 was expressed by a few AMs, type II pneumocytes and perivascular smooth muscle cells. CWP sections were characterized by an increased number of AMs and mainly by the presence of fibroblasts (in the myogenic area of fibrotic lesions) and hyperplastic type II pneumocytes, which were strongly immunostained for MCP-1. Our data demonstrate that: 1) patients with coal worker's pneumoconiosis have a marked pulmonary overproduction of monocyte chemoattractant protein-1; and 2) in addition to alveolar macrophages, fibroblasts (probably myofibroblasts) and hyperplastic type II pneumocytes may also be responsible for this increased level of monocyte chemoattractant protein-1 in coal worker's pneumoconiosis. %U //www.qdcxjkg.com/content/erj/10/3/557.full.pdf