@Article {Kim542，作者= {Kim，KC和Park，HR和Shin，Cy and Akiyama，T和KO，KH}，title = {核苷酸诱导的粘蛋白从主要的仓鼠气管表面上皮细胞中释放，涉及P2U Purinoccepor}，音量= {9}，number = {3}，pages = {542--548}，ear = {1996}，publisher = {欧洲呼吸社会}，摘要188bet官网地址= {由气道表面上皮细胞释放，由细胞外腺苷调节三磷酸（ATP）通过P2 Purinoceptor介导的机制。本实验的目的是检查三磷酸尿苷（UTP）可能参与此嘌呤能信号转导途径。使用原发性仓鼠气管表面上皮细胞，比较了ATP和UTP的能力：1）将ATP伽玛S35结合置换为完整的细胞；2）积累肌醇磷酸盐；3）刺激粘蛋白释放。最后，检查了P2U受体信息的存在。我们的结果表明：1）UTP在置换ATP伽马S35结合（中值抑制浓度（IC50）240 vs 2.9 microM中，UTP的效率要比ATP差得多。2）在积累肌醇磷酸盐（100 vs 43 \％时，在2mm时增加100 vs），UTP比ATP更有效； 3) UTP was equipotent with ATP in stimulating mucin release; 4) Northern blot analysis of messenger ribonucleic acids (mRNAs) with a mouse P2u receptor complementary deoxyribonucleic acid (cDNA) probe revealed a single specific band (2.8 kb), partial sequencing of which showed a great homology with those of human or mouse P2u receptors. We conclude that, although both ATP and UTP are equipotent in stimulating mucin release, their binding kinetics to the cell surface are quite different, suggesting the presence of a common binding domain which may be responsible for the mucin release by these nucleotides. We suggest that the P2u purinoceptor is likely to be responsible for mucin release by these nucleotides, probably via activation of phospholipase C.}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/9/3/542}, eprint = {//www.qdcxjkg.com/content/9/3/542.full.pdf}, journal = {European Respiratory Journal} }