T1诱导的痰细胞和炎症液相指标:比较治疗二硫苏糖醇与磷酸盐JF -欧洲呼吸杂志》乔和J SP - 1336 LP - 1340欧元六世- 10 - 6盟Efthimiadis盟——Pizzichini MM盟——Pizzichini E盟——Dolovich J盟——Hargreave FE Y1 - 1997/06/01 UR - //www.qdcxjkg.com/content/10/6/1336.abstract N2 -治疗痰与二硫苏糖醇(DTT)提供可靠的测量细胞和流体相气道炎症的标志。我们研究了与磷酸盐缓冲盐水(PBS)相比，DTT处理对这些测量的影响程度。在2小时内对20例哮喘患者的高渗盐水诱导痰进行检查。从痰液中收集比唾液更固体(更少液体)的部分。选择的痰液被分成两部分:一组用一体积的DTT和一体积的PBS处理，另一组用两体积的PBS处理。对滤液进行盲法检测，包括细胞总数和差异计数、生存能力、液体期嗜酸性粒细胞阳离子蛋白(ECP)、纤维蛋白原、白细胞介素(IL)-5和IL-8。与PBS相比，DTT处理的痰中活细胞比例较低(中位数为66比74%;p = 0.003)。相比之下，经dtt处理的痰细胞总数更高(中位数为8.8 vs 2.8 x 10(6) mL(-1);ECP水平(中位数1340 vs 584 mg x L(-1); p<0.001) The measurements were similar with respect to the proportion of eosinophils, neutrophils, lymphocytes, macrophages, and fluid-phase fibrinogen, IL-5 and IL-8. We conclude that dithiothreitol disperses cells more effectively and that this might account for the higher levels of eosinophil cationic protein. Dithiothreitol may affect cell viability, but the changes are not relevant with respect to cell counts. Additionally, dithiothreitol does not seem to influence the other measurements performed. ER -