RT期刊文章SR电子T1诱导的痰细胞和流体相炎症指数：与二硫代硫醇与磷酸盐缓冲盐水JF欧洲呼吸杂志的治疗比较188bet官网地址A A1 Pizzichini，MM A1 Pizzichini，E A1 Dolovich，J A1 Hargreave，Fe Yr 1997 ul //www.qdcxjkg.com/content.com/content/10/6/1336.Abstract用Dithiothiothreitol（dtt）对尖峰进行治疗气道炎症的细胞和流体相标记的测量。我们研究了与磷酸盐缓冲盐水（PBS）相比，DTT处理影响这些测量的程度。在2小时内检查了从20名哮喘患者中收集的高渗盐水诱导的痰液。从期望值中收集了比唾液更坚固（流体少）的所有部分。然后将选定的痰液分为两部分：一部分用一卷DTT和一个PBS处理，另一个带有两卷PBS。评估滤膜的总细胞计数，生存力和流体嗜酸性粒细胞阳离子蛋白（ECP），纤维蛋白原，白介素（IL）-5和IL-8。与PBS相比，用DTT处理的痰液具有较低的活细胞比例（中位数为66 vs 74％； P = 0.003）。相比之下，DTT处理的痰液的总细胞计数较高（中值8.8 vs 2.8 x 10（6）ml（-1）； P <0.001）和ECP水平（中位1340 vs 584 mg x L（-1）； P<0.001) The measurements were similar with respect to the proportion of eosinophils, neutrophils, lymphocytes, macrophages, and fluid-phase fibrinogen, IL-5 and IL-8. We conclude that dithiothreitol disperses cells more effectively and that this might account for the higher levels of eosinophil cationic protein. Dithiothreitol may affect cell viability, but the changes are not relevant with respect to cell counts. Additionally, dithiothreitol does not seem to influence the other measurements performed.