宋盛仁付振利关瑞娟赵杰杨鹏辉李阳尹航赖航龚云欣赵根成余思敏彭小敏何颖钟玉梅钟南山苏云欣特发性肺纤维化(Idiopathic pulmonary fibrosis, IPF)是一种致命的肺部疾病，治疗选择很少。开发抗ipf策略的不成功促使研究人员重新考虑选择动物模型和评估方法的重要性。目前，博莱霉素诱导的肺纤维化小鼠模型是否最终恢复到正常还没有定论。本研究旨在通过结合最新技术(micro-CT成像和降解胶原的组织学检测)和传统方法，跟踪BLM(博莱霉素)治疗延长时间小鼠肺的动态纤维化特征。此外，我们还应用免疫组化技术来研究所有含羟脯氨酸分子的分布。经经典生化法测定，肺总羟脯氨酸含量在博莱霉素损伤后4周达到峰值，并在此之后一直维持较高水平，直到实验结束(16周)。这一结果似乎与其他纤维化评价指标的变化存在部分矛盾，纤维化峰值后胶原蛋白逐渐降解，肺通气恢复。我们的抗羟脯氨酸免疫染色和抗降解胶原的荧光肽染色数据很好地调和了这种不一致，这些数据显示，在10周或16周时，大量富含羟脯氨酸的降解胶原碎片在细胞内区域滞留和富集，而不是在blm治疗后的4周。 Hence, our present data not only offer respiratory researchers a new perspective towards the resolution nature of mouse lung fibrosis, but also remind them to be cautious while using hydroxyproline content assay to evaluate the severity of fibrosis.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Shengren Song has nothing to disclose.Conflict of interest: Zhenli Fu has nothing to disclose.Conflict of interest: Ruijuan Guan has nothing to disclose.Conflict of interest: Jie Zhao has nothing to disclose.Conflict of interest: Penghui Yang has nothing to disclose.Conflict of interest: Yang Li has nothing to disclose.Conflict of interest: Hang Yin has nothing to disclose.Conflict of interest: Yunxin Lai has nothing to disclose.Conflict of interest: Gencheng Gong has nothing to disclose.Conflict of interest: Simin Zhao has nothing to disclose.Conflict of interest: Jiangtian Yu has nothing to disclose.Conflict of interest: Xiaomin Peng has nothing to disclose.Conflict of interest: Ying He has nothing to disclose.Conflict of interest: Yumei Luo has nothing to disclose.Conflict of interest: Nanshan Zhong has nothing to disclose.Conflict of interest: Jin Su has nothing to disclose. %U //www.qdcxjkg.com/content/erj/early/2021/09/16/13993003.00864-2021.full.pdf