Ty-jour t1 - 细胞内羟脯氨酸印迹后源霉素诱导的肺纤维化JF - 欧洲呼吸杂志Jo - Eur Respir J Do - 10.1183 / 13993003 - 2100864-2021 SP - 2100864 Au - Song，Shengren Au - Fu，Zhenli Au - Guan，瑞娟·奥·赵，杰·奥 - 杨，澎湖·李，杨··尹，恒武，云信坝 - 龚，葛城坝，思in·苏，江天奥 - 彭，萧代·鲍 - 他，莹奥 - 罗，yumei au - zhong，nanshan au - su，金y1 - 2021/01/01 ur - //www.qdcxjkg.com/content/early/2021/09/16/13993003.00864-2021.AbstractN2 - 特发性肺纤维化（IPF）是一种致命的肺病，治疗方案很少。制定反IPF战略的糟糕成功使研究人员重新考虑了动物模型和评估方法的首选的重要性。目前，尚未解决博霉素诱导的肺纤维化小鼠模型最终恢复到分辨率。本研究旨在通过最新技术的组合遵循BLM（BLEOMICIN） - 治疗小鼠肺的动态纤维化特征（具有传统方法的微型CT成像和降解胶原蛋白的组织学检测。此外，我们还应用免疫组织化学来探讨所有含羟脯氨酸的分子的分布。通过经典生化方法确定，肺霉素损伤后4周达到峰的总肺羟脯氨酸含量，此后保持稳定的高水平直至其结束实验（16周）。这种结果似乎部分地与其他纤维化评估参数的变化部分矛盾，这表明胶原蛋白的逐渐降解和肺曝气术后的血栓峰恢复。从免疫染色的免疫染色和荧光肽染色的荧光肽染色的数据与降解胶原蛋白的荧光肽染色的数据进行很好地调整，并在10-或16周，而不是在植物中显示大量羟脯氨酸富含降解的胶原碎片和富含细胞内区域内的溶质和富集 4-week post the BLM-treatment. Hence, our present data not only offer respiratory researchers a new perspective towards the resolution nature of mouse lung fibrosis, but also remind them to be cautious while using hydroxyproline content assay to evaluate the severity of fibrosis.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Shengren Song has nothing to disclose.Conflict of interest: Zhenli Fu has nothing to disclose.Conflict of interest: Ruijuan Guan has nothing to disclose.Conflict of interest: Jie Zhao has nothing to disclose.Conflict of interest: Penghui Yang has nothing to disclose.Conflict of interest: Yang Li has nothing to disclose.Conflict of interest: Hang Yin has nothing to disclose.Conflict of interest: Yunxin Lai has nothing to disclose.Conflict of interest: Gencheng Gong has nothing to disclose.Conflict of interest: Simin Zhao has nothing to disclose.Conflict of interest: Jiangtian Yu has nothing to disclose.Conflict of interest: Xiaomin Peng has nothing to disclose.Conflict of interest: Ying He has nothing to disclose.Conflict of interest: Yumei Luo has nothing to disclose.Conflict of interest: Nanshan Zhong has nothing to disclose.Conflict of interest: Jin Su has nothing to disclose. ER -