TY -的T1 -肺炎支原体马车evades induction of protective mucosal antibodies JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00129-2021 SP - 2100129 AU - de Groot, Ruben Cornelis Anthonie AU - Cristina Estevão, Silvia AU - Meyer Sauteur, Patrick Michael AU - Perkasa, Aditya AU - Hoogenboezem, Theo AU - Spuesens, Emiel Benny Margriet AU - Verhagen, Lilly Maria AU - van Rossum, Anna Maria Christiane AU - Unger, Wendy Wilhelmina Josephina Y1 - 2021/01/01 UR - //www.qdcxjkg.com/content/early/2021/08/12/13993003.00129-2021.abstract N2 - Mycoplasma pneumoniae is the most common bacterial cause of pneumonia in children hospitalised for community-acquired pneumonia. Prevention of infection by vaccines may be an important strategy in the presence of emerging macrolide resistant M. pneumoniae. However, knowledge of immune responses to M. pneumoniae is limited, complicating vaccine design. We therefore studied the antibody response during M. pneumoniae infection and asymptomatic carriage.In a nested case-control study (n=80) of M. pneumoniae carriers and matched controls we observed that carriage by M. pneumoniae does not lead to a rise in either mucosal or systemic M. pneumoniae-specific antibodies, even after months of persistent carriage. We replicated this finding in a second cohort (n=69) and also found that during M. pneumoniae community-acquired pneumonia, mucosal levels of M. pneumoniae-specific IgA and IgG did increase significantly. In vitro adhesion assays revealed that high levels of M. pneumoniae-specific antibodies in nasal secretions of paediatric patients prevented the adhesion of M. pneumoniae to respiratory epithelial cells.In conclusion, our study demonstrates that M. pneumoniae-specific mucosal antibodies protect against bacterial adhesion to respiratory epithelial cells and are induced only during M. pneumoniae infection and not during asymptomatic carriage. This is strikingly different from carriage with bacteria such as Streptococcus pneumoniae where mucosal antibodies are induced by bacterial carriage.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Ruben CornelisConflict of interest: Dr. de Groot has nothing to disclose.Conflict of interest: Silvia CristinaConflict of interest: Dr. Estevão has nothing to disclose.Conflict of interest: Dr. Meyer Sauteur has nothing to disclose.Conflict of interest: Dr. Perkasa has nothing to disclose.Conflict of interest: Dr. Hoogenboezem has nothing to disclose.Conflict of interest: Dr. Spuesens has nothing to disclose.Conflict of interest: Dr. Verhagen has nothing to disclose.Conflict of interest: Dr. van Rossum has nothing to disclose.Conflict of interest: Dr. Unger has nothing to disclose. ER -