PT-日记文章Au -Sulaiman，Imran Au -Wu -Wu，Benjamin G. Au -Li，Yonghua au -tsay -Tsay，Jun -Chieh au -Sauthoff，Maya au -Scott -Scott，Adrienne S. au -Ji -JI，JI，Kun Au -Kun Au -Koralov，Sergeii，Sergeii，Sergeii，Sergeii，SergeiiB. au -Weiden，Michael Au -Clemente，Jose C. Au -Jones，Drew Au -Huang，Yvonne J. Au -Stringer，Kathleen A. Au -Zhang -Zhang -Zhang，Lingdi Au -Geber -Geber -Geber，Adam Au -Banakis，Banakis，Stephanie au -au--Tipton，Laura au -Ghedin，Elodie au -Segal，Leopoldo N. Ti-微生物组的功能性下气道基因组分析，以捕获主动微生物代谢辅助辅助-101183/13993003.034343434-2020vi -58 ip -1 4099 -http：//www.qdcxjkg.com/content/58/1/1/2003434.short 4100 -http：//www.qdcxjkg.com/content.com/content/58/1/1/1/2003434.floEUR RESSIR J2021 7月1日;58 AB - 基于细菌16S rRNA基因测序的下气道的背景微生物组研究评估微生物群落结构，但只能推断功能特征。较低气道中的微生物产物，例如短链脂肪酸（SCFA），对宿主的免疫张力产生了重大影响。因此，需要进行微生物组分析的功能方法。在这里，我们使用了研究支气管镜吸烟者队列中的上和下气道样品。此外，我们在实验小鼠模型中验证了结果。我们通过使用全基因组shot弹枪（WGS）和RNA metatranscriptome测序来扩展了微生物群的表征超过16S rRNA基因测序。 SCFAs were also measured in lower airway samples and correlated with each of the sequencing datasets. In the mouse model, 16S rRNA gene and RNA metatranscriptome sequencing were performed.Results Functional evaluations of the lower airway microbiota using inferred metagenome, WGS and metatranscriptome data were dissimilar. Comparison with measured levels of SCFAs shows that the inferred metagenome from the 16S rRNA gene sequencing data was poorly correlated, while better correlations were noted when SCFA levels were compared with WGS and metatranscriptome data. Modelling lower airway aspiration with oral commensals in a mouse model showed that the metatranscriptome most efficiently captures transient active microbial metabolism, which was overestimated by 16S rRNA gene sequencing.Conclusions Functional characterisation of the lower airway microbiota through metatranscriptome data identifies metabolically active organisms capable of producing metabolites with immunomodulatory capacity, such as SCFAs.This study shows that both whole-genome shotgun and RNA metatranscriptome sequencing can be done on lower airway samples and can provide valuable information on bacterial function https://bit.ly/3hNmZfi