%0期刊文章%A Lepzien, Rico %A Liu, Sang %A Czarnewski, Paulo %A Nie, Mu %A Österberg, Björn %A Baharom, Faezzah %A Pourazar, Jamshid %A Rankin, Gregory %A Eklund, Anders %A Bottai, Matteo %A Kullberg, Susanna %A Blomberg, Anders %A Grunewald, Johan %A Smed-Sörensen，肺结节病中的单核细胞是有效的肿瘤坏死因子产生者，可预测疾病结局J欧洲呼吸杂志P 2003468 %V 58 %N 1 %X背景肺结节病是一种以肉芽肿形成和不同临床结局为特征的炎症性疾病。肿瘤坏死因子(TNF)是一种促进肉芽肿形成的促炎细胞因子，高水平的TNF已被证明与进展性疾病有关。单核吞噬细胞(MNPs)是TNF的有效生产者，对炎症反应高度敏感。在结节病中，肺泡巨噬细胞已被很好地研究。然而，MNPs还包括单核细胞/单核细胞来源的细胞和树突状细胞，尽管它们在炎症中起着核心作用，但在结节病中研究很少。目的探讨肺单核细胞来源细胞和树突状细胞在结节病发病中的作用。方法我们对108例结节病患者和30例健康对照的血液和支气管肺泡灌洗液中的MNP亚群进行了深入的表型、功能和转录组分析。我们跟踪了患者的临床发展，并评估了诊断时MNP亚群的库和功能与2年疾病结局的相关性。结果结节病患者血液中单核细胞/单核细胞源性细胞及BAL较健康对照组增加。有趣的是，诊断时血液中间单核细胞的高频率与慢性疾病的发展有关。RNA测序分析显示结节病患者BAL中MNPs高度炎性。 Furthermore, frequencies of BAL monocytes/monocyte-derived cells producing TNF without exogenous stimulation at time of diagnosis increased in patients that were followed longitudinally. In contrast to alveolar macrophages, the frequency of TNF-producing BAL monocytes/monocyte-derived cells at time of diagnosis was highest in sarcoidosis patients that developed progressive disease.Conclusion Our data show that pulmonary monocytes/monocyte-derived cells are highly inflammatory and can be used as a predictor of disease outcome in sarcoidosis patients.Phenotypic, transcriptomic and functional mapping of blood and pulmonary mononuclear phagocytes in sarcoidosis patients found that frequency and function of pulmonary monocytes at time of diagnosis predict 2-year outcome in sarcoidosis https://bit.ly/2JX8fhr %U //www.qdcxjkg.com/content/erj/58/1/2003468.full.pdf