TY - JOUR T1 - 在人类的精确切割肺组学时代的曙光切片JF - 欧洲呼吸杂志JO - EUR呼吸杂志DO - 10.1183 / 13993003.00203-2021 VL - 58 - 1个SP - 2100203 AU - Stegmayr，约翰AU- 瓦格纳，达西E. Y1 - 2021年7月1日UR - //www.qdcxjkg.com/content/58/1/2100203.abstract N2 - 精确切肺片（PCLS）已在生物医学研究中使用for over 50 years (30 years for human PCLS), but the majority of initial work focused on toxicology, tissue mechanics, and modelling airway responses for asthma [1–3]. In recent years, PCLS have been increasingly used for studying acute and chronic lung diseases at the molecular level, as well as for evaluating therapeutics [3, 4]. In particular, there is high interest in using PCLS derived from human lungs, due to the ability to directly study species-specific effects in human tissue and because they better capture clinical heterogeneity (e.g. age, broader spectrum of disease status, or the presence of other co-morbidities). However, one of the major difficulties hampering the further use of this model system has been due to difficulties in directly applying new and emerging state-of-the-art analysis methods to PCLS, including for example mass spectrometry, next-generation sequencing, and advanced microscopy. The study by Khan et al. [5] in this issue of the European Respiratory Journal details multiple technical advances and makes a significant leap forward in our ability to perform advanced and detailed molecular analysis of human PCLS-based disease models.New techniques permit the use of multiple omics and advanced imaging technologies with human precision-cut lung slices (PCLS) to allow for more objective and comprehensive molecular analysis to bring PCLS closer to the era of precision medicine https://bit.ly/3deBjx2 ER -