TY - Jour T1 - OMICS时代的人类精密切割肺切片JF - 欧洲呼吸杂志Jo - Eur Respir J Do - 10.1183 / 13993003.00203-2021 VL - 58是 - 1 SP - 2100203 Au - Stegmayr，John Au- 瓦格纳，达西·y. y1 - 2021/07/01 UR - //www.qdcxjkg.com/content/58/1/2100203.Abstract n2 - 精密切割肺切片（PCLS）已用于生物医学研究超过50年（人类PCLS 30年），但大多数初始工作都集中在毒理学，组织力学和哮喘造型气道反应的初始工作[1-3]。近年来，PCLS越来越多地用于研究分子水平的急性和慢性肺部疾病，以及评估治疗方法[3,4]。In particular, there is high interest in using PCLS derived from human lungs, due to the ability to directly study species-specific effects in human tissue and because they better capture clinical heterogeneity (e.g. age, broader spectrum of disease status, or the presence of other co-morbidities). However, one of the major difficulties hampering the further use of this model system has been due to difficulties in directly applying new and emerging state-of-the-art analysis methods to PCLS, including for example mass spectrometry, next-generation sequencing, and advanced microscopy. The study by Khan et al. [5] in this issue of the European Respiratory Journal details multiple technical advances and makes a significant leap forward in our ability to perform advanced and detailed molecular analysis of human PCLS-based disease models.New techniques permit the use of multiple omics and advanced imaging technologies with human precision-cut lung slices (PCLS) to allow for more objective and comprehensive molecular analysis to bring PCLS closer to the era of precision medicine https://bit.ly/3deBjx2 ER -