Ty -jour t1-转录组研究揭示了人类肺移植物中的供体特异性基因特异性基因特异性基因签名JF-欧洲呼吸杂志JO -EUR RESSIR J DO -10.1183/13993003.00327-2020-2020 VL -57 IS -4 SP -20000327 AU -BACIU，CRISTINA AU -BACISINA AU -BACISINA AU -BACISINA AU -BACISINA AU--Sage, Andrew AU - Zamel, Ricardo AU - Shin, Jason AU - Bai, Xiao-Hui AU - Hough, Olivia AU - Bhat, Mamatha AU - Yeung, Jonathan C. AU - Cypel, Marcelo AU - Keshavjee, Shaf AU - Liu，mingyao y1-2021/04/01 ur -http：//www.qdcxjkg.com/content/57/4/4/2000327.abstract N2-简介循环死亡后捐赠中肺部肺部的肺部（DCD）外（DCD之后）的捐赠后，大脑后捐赠后死亡（DBD）在全球范围内变得常规，以解决全球器官短缺。用于供体肺部评估和修复的体内肺灌注（EVLP）的发展有助于增加DCD肺的使用。我们假设对DBD和DCD供体之间的肺之间的差异以及EVLP和直接移植（非EVLP）肺之间的差异有更好的理解，将导致发现供体肺部修复和重新调节的损伤目标。在冷缺血时间结束时收集了来自人类DBD（n = 177）和DCD（n = 65）供体肺的活检（n = 65）供体肺。所有样品均使用微阵列测定法处理。使用R，Ingenuity途径分析和字符串进行基因表达，网络和途径分析。通过蛋白质测定，多个逻辑回归和10倍交叉验证来验证结果。分析表明，来自DBD供体的肺有炎症细胞因子和途径的上调。 In contrast, DCD lungs display a transcriptome signature of pathways associated with cell death, apoptosis and necrosis. Network centrality revealed specific drug targets to rehabilitate DBD lungs. Moreover, in DBD lungs, tumour necrosis factor receptor-1/2 signalling pathways and macrophage migration inhibitory factor-associated pathways were activated in the EVLP group. A panel of genes that differentiate the EVLP from the non-EVLP group in DBD lungs was identified.Conclusion The examination of gene expression profiling indicates that DBD and DCD lungs have distinguishable biological transcriptome signatures.Lungs from DBD donors have increased activation of inflammatory pathways. In contrast, cell death, apoptosis and necrosis are activated in lungs from DCD donors. EVLP and non-EVLP lungs also have distinct transcriptomic signatures. https://bit.ly/36SWsdb ER -