TY - JOUR T1 - 单核吞噬细胞系统有助于移植后闭塞性细支气管炎纤维化JF - 欧洲呼吸杂志JO - EUR呼吸杂志DO - 10.1183 / 13993003.00344-2020 VL - 57 - 3 SP - 2000344 AU - 迪坎普利，玛丽亚-Pia AU - 阿祖兹，阿卜杜勒卡德尔·AU - Assabban，Assiya AU - Scaillet，杰西卡AU - Splittgerber，马里昂AU - 范Keymeulen，亚历山德拉AU - LIBERT，弗雷德里克AU - Remmelink，和Myriam AU - 乐莫因，阿兰AU - 勒梅特，菲利普AU- Goriely，斯坦尼斯Y1 - 2021年3月1日UR - //www.qdcxjkg.com/content/57/3/2000344.abstract N2 - 闭塞性细支气管炎综合征（BOS）是纤维化疾病是对重负责肺移植后死亡率很高。肌成纤维细胞这纤维化过程的主要效应，但它们的起源仍有争议。这项工作的目的是确定负责移植后气道成纤维obliteration.Lineage跟踪工具间充质细胞被用来追踪前体或异位气管移植模型肌成纤维细胞的贫化的潜在来源。同种异体移植物进行组织学，共聚焦显微镜，流式细胞仪或单细胞转录分析进行分析。BOS外植体通过组织学和同种异体移植物的共聚焦microscopy.Myofibroblasts进行评价受者来源。当接受者小鼠用他克莫司治疗的，我们观察到罕见上皮 - 间质转化现象和在供体来源肌成纤维细胞（p值= 0.0467）的总体增加，但是这些细胞的比例维持在低水平（7％）。造血细胞，以及特别是单核吞噬细胞系统，给人们带来的阻塞气道中发现的多数肌成纤维细胞。CX3CR1 +细胞的消融降低成纤维闭塞（p值= 0.0151）和肌成纤维细胞积累（p值= 0.0020）。 Single-cell RNA sequencing revealed similarities between myeloid-derived cells from allografts and both murine and human samples of lung fibrosis. Finally, myofibroblasts expressing the macrophage marker CD68 were increased in BOS explants when compared to controls (14.4% versus 8.5%, p=0.0249).Recipient-derived myeloid progenitors represent a clinically relevant source of mesenchymal cells infiltrating the airways after allogeneic transplantation. Therapies targeting the mononuclear phagocyte system could improve long-term outcomes after lung transplantation.Bronchiolitis obliterans syndrome remains a major cause of mortality after lung transplantation. The mononuclear phagocyte system actively contributes to fibrotic occlusion of rejected airways, thereby representing a promising novel therapeutic target. https://bit.ly/3hQh8UL ER -