TY-JUR T1 - 过敏原产生与气道功能障碍相关的促炎氧化磷脂酰胆碱JF - 欧洲呼吸杂志Jo - EUR RESPIR J DO - 10.11839-2020 VL - 57是 - 2 SP - 2000839 Au - Pascoe，Christopher D.Au - Jha，Aruni Au - Ryu，Min Hyung Au - Ragheb，Mirna Au - vaghasiya，Jignesh Au - Basu，Sujata Au - Stelmack，Gerald L. Au - Srinathan，Sadeesh Au - Kinane，Biniam Au - Kindrachuk，Jason Au -O'Byrne，Paul M. Au - Gauvreau，Gail M. Au - Ravandi，Amir Au - Carlsten，Christopher Au - Halayko，Andrew J. Y1 - 2021/02/01 UR - //www.qdcxjkg.com/含量/ 57/2 / 2000839.抽象N2-氧化磷脂酰胆碱（OXPC）在氧化胁迫升高的条件下生产，并且可以有助于人类疾病病理学。然而，它们在过敏性哮喘中的作用是未开发的。本研究的目的是在气道高反应性（AHR）或轻度哮喘的攻击后的过敏原挑战后的气道中的oxpc型材。还将确定oxpcs与哮喘相关的病理生物学的能力。使用来自两个人队列的支气管肺泡灌洗液，使用超高效液相色谱 - 串联质谱法定量oxpc物种。鼠薄切割肺切片用于测量由OXPC引起的气道缩小。人体气道平滑肌（HASM）细胞暴露于OXPC，以评估炎症表型的浓度相关变化，并激活信号通信网络中的信号线曲线在有和没有AHR的人之间不同，与甲胆碱反应性相关。将患有轻度哮喘的患者暴露于过敏原制作的独特oxpc签名，与晚期哮喘反应的严重程度相关。 OxPCs dose-dependently induced 15% airway narrowing in murine thin-cut lung slices. In HASM cells, OxPCs dose-dependently increased the biosynthesis of cyclooxygenase-2, interleukin (IL)-6, IL-8, granulocyte−macrophage colony-stimulating factor and the production of oxylipins via protein kinase C-dependent pathways.Data from human cohorts and primary HASM cell culture show that OxPCs are present in the airways, increase after allergen challenge and correlate with metrics of airway dysfunction. Furthermore, OxPCs may contribute to asthma pathobiology by promoting airway narrowing and inducing a pro-inflammatory phenotype and contraction of airway smooth muscle. OxPCs represent a potential novel target for treating oxidative stress-associated pathobiology in asthma.Unique profiles of oxidised phospholipids in the human lung correlate with airway pathophysiology. They are novel pro-inflammatory mediators with direct effects in structural cells via complex pathways, and are not targeted by standard asthma therapies. https://bit.ly/34UO2AL ER -