TY -的T1 -可交叉反应的抗体ust mite-derived enolase induce neutrophilic airway inflammation JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.02375-2019 VL - 57 IS - 1 SP - 1902375 AU - Lin, Jianli AU - Huang, Nana AU - Li, Jing AU - Liu, Xiaoyu AU - Xiong, Qing AU - Hu, Chengshen AU - Chen, Desheng AU - Guan, Lvxin AU - Chang, Kexin AU - Li, Dan AU - Tsui, Stephen Kwok-Wing AU - Zhong, Nanshan AU - Liu, Zhigang AU - Yang, Ping-Chang Y1 - 2021/01/01 UR - //www.qdcxjkg.com/content/57/1/1902375.abstract N2 - Background and aims Neutrophilic inflammation is a hallmark of some specific asthma phenotypes; its aetiology is not yet fully understood. House dust mite (HDM) is the most common factor in the pathogenesis of airway inflammation. This study aims to elucidate the role of cross-antibodies against HDM-derived factors in the development of neutrophilic inflammation in the airway.Methods Blood samples were collected from asthma patients with chronic neutrophilic asthma for analysis of HDM-specific cross-reactive antibodies. The role of an antibody against HDM-derived enolase (EnoAb) in the impairment of airway epithelial barrier function and induction of airway inflammation was assessed in a cell culture model and an animal model.Results High similarity (72%) of the enolase gene sequences was identified between HDM and human. Serum EnoAb was detected in patients with chronic neutrophilic asthma. The EnoAb bound to airway epithelial cells to form complexes with enolase, which activated complement, impaired airway epithelial barrier functions and induced neutrophilic inflammation in the airway tissues.Conclusions HDM-derived enolase can induce specific cross-antibodies in humans, which induce neutrophilic inflammation in the airway.House dust mite (HDM)-derived enolase induces cross-antibodies in the body, which recognise both HDM-derived enolase and the enolase in the airway epithelial cells, activate complement and induce neutrophilic inflammation in the airway tissues https://bit.ly/2XKSCgy ER -