% 0期刊文章%一个广州市李%江Weijia % Junfei黄%任一个Lijuan刘英% %一个李庄% Qinni郑%一个明王%鲁伊·杨%易曾庆红%易王% T高度敏感和特定的诊断COVID-19通过逆转录多个cross-displacement amplification-labelled纳米生物传感器% D J 2020% R 10.1183/13993003.02060 -2020%欧洲呼吸杂志% P 2002060 % V 56% N 6% X背景小说人类冠状病毒疫情的严重急性呼吸系统综合症冠状病毒2 (SARS-CoV-2)(也称为2019 - ncov)已成为一个全球性的健康问题。迫切需要快速和易于使用的诊断技术诊断SARS-CoV-2感染。方法:我们设计了一个逆转录多cross-displacement放大(RT-MCDA)加上nanoparticle-based生物传感器测定(RT-MCDA-BS)快速、敏感和特定的诊断冠状病毒疾病2019 (COVID-19)。两套引物设计目标的开放阅读框1 a / b和SARS-CoV-2核蛋白基因。总共183份临床样本,其中包括65名患者COVID-19感染和118例其他病原体感染被用来证明试验的可行性。诊断结果报告视觉使用生物传感器。发现试验设计进行了使用一个简单的仪器可以反应在一个恒定的温度维持在64°C只有35分钟。总COVID-19 RT-MCDA-BS测试程序可在1 h。的COVID-19 RT-MCDA-BS可以检测到目标序列的5份。COVID-19患者65份临床样本中,22例(33.8%)阳性结果从粪便,鼻、咽和肛门拭子通过COVID-19 RT-MCDA-BS化验,而实时一试验仅检测20例(30.7%)阳性结果在这些样品。 No positive results were obtained from clinical samples with non-COVID-19 infections.Interpretation COVID-19 RT-MCDA-BS was a rapid, reliable, low-cost and easy-to-use assay, which could provide an attractive laboratory tool to diagnose COVID-19 in multiple clinical specimens, especially for field, clinic laboratories and primary care facilities in resource-poor settings.We devised a novel method (COVID-19 RT-MCDA-BS) to diagnose COVID-19. Its rapidity, low cost and ease of use make the method an ideal tool for use in field, primary and clinical laboratories, especially for resource-poor settings. https://bit.ly/3j08CU2 %U //www.qdcxjkg.com/content/erj/56/6/2002060.full.pdf