TY -的T1 Epigenome-wide协会研究DNA甲基化和成人哮喘在农业肺部健康研究摩根富林明-欧洲呼吸杂志》乔和J - 10.1183/13993003.00217 -2020欧元六世- 56 - 3 SP - 2000217 AU - Thanh t .黄平君盟Sinjini Sikdar盟,陈健徐盟Jonathan Cardwell - Mi Kyeong李盟盟-埃里克《盟美狄亚Imboden AU -小桢盟anne - marie Madore AU -康康舞七盟天元王盟——布莱恩·d·班尼特盟——詹姆斯·m·沃德AU -克里斯汀·g .公园盟劳拉e . Beane-Freeman盟Debra王盟-艾莉森Motsinger-Reif盟David m . Umbach AU - Annah b . Wyss盟David a . Schwartz盟拉普莱斯凯瑟琳-胡安·c·青瓷盟盟-卡罗尔欧博盟妮可Probst-Hensch盟伊凡娜杨诉AU -杰拉德·h·Koppelman盟斯蒂芬妮·J·伦敦Y1 - 2020/09/01 UR - //www.qdcxjkg.com/content/56/3/2000217.abstract N2 - Epigenome-wide甲基化的研究支持儿童哮喘的表观遗传机制的作用;然而,研究成人很少见,很少有研究non-atopic哮喘。我们进行了最大epigenome-wide协会研究(ewa)的血液DNA甲基化在成人关系non-atopic和过敏性哮喘。我们测量血液中DNA甲基化使用Illumina公司MethylationEPIC数组中当前病例对照研究的2286名参与者成人哮喘嵌套在美国农业队列。异位性被定义为特定血清免疫球蛋白E (IgE)。参与者被归类为特异反应性没有哮喘(n = 185), non-atopic哮喘(n = 673)、过敏性哮喘(n = 271),或者一个参考群特异反应性和哮喘(n = 1157)。使用逻辑回归进行了分析。没有观察到协会有特异反应性没有哮喘。无数cytosine-phosphate-guanine (CpG)网站有差异甲基化在non-atopic哮喘(八在family-wise错误率(弗雷德里克)术;9×10−8,524错误发现率(罗斯福)小于0.05),与382年的小说《基因。更多CpG网站被确定在过敏性哮喘(181年弗兰克-威廉姆斯,1086年罗斯福),与569年的小说《基因。 104 FDR CpG sites overlapped. 35% of CpG sites in non-atopic asthma and 91% in atopic asthma replicated in studies of whole blood, eosinophils, airway epithelium, or nasal epithelium. Implicated genes were enriched in pathways related to the nervous system or inflammation.We identified numerous, distinct differentially methylated CpG sites in non-atopic and atopic asthma. Many CpG sites from blood replicated in asthma-relevant tissues. These circulating biomarkers reflect risk and sequelae of disease, as well as implicate novel genes associated with non-atopic and atopic asthma.Distinct methylation signals are found in non-atopic and atopic asthma. Most are related to gene expression and are replicated in asthma-relevant tissues, confirming the value of blood DNA methylation for identifying novel genes linked in asthma pathogenesis. https://bit.ly/2VnbJg3 ER -