TY - JOUR T1 -肺内气道平滑肌高反应与哮喘中不同的蛋白质组JF -欧洲呼吸杂志JO - Eur Respir J DO - 10.1183/13993003.02178-2019 VL - 56 is - 1 SP - 1902178 AU - Ijpma, Gijs AU - Kachmar, Linda AU - Panariti, Alice AU - Matusovsky, Oleg S. AU - toorgerson, Dara AU - Benedetti, Andrea AU - Lauzon，Anne-Marie Y1 - 20/07/01 UR - //www.qdcxjkg.com/content/56/1/1902178.abstract N2 -哮喘加重期气道收缩是气道平滑肌(ASM)收缩的结果。虽然人们普遍认为哮喘的ASM是过度收缩的，但这并没有得到明确的证明。气道高反应性(AHR)是单独增加ASM质量的结果还是单位肌肉产生的收缩力的增加直接决定了治疗的潜在途径。为了评估ASM是否具有高收缩性，我们对从肺内气道和气管分离的ASM进行了一系列力学测量。我们分析了ASM和整个气道的蛋白质组，以验证蛋白质组的变化是否有助于ASM性质的变化。我们报告了哮喘患者肺内支气管(哮喘患者气道阻力增加的部位)特异的孤立ASM收缩应力和僵硬度的增加。其他收缩参数没有改变。无偏质谱数据的主成分分析(PCA)显示哮喘受试者在ASM特异性蛋白方面有明显的聚类。全气道蛋白质组结构蛋白表达上调。我们没有发现任何证据表明哮喘性ASM中肌凝蛋白活性的调节存在差异。In conclusion, we showed that ASM is indeed hyperreactive at the level of intrapulmonary airways in asthma. We identified several proteins that are upregulated in asthma that could contribute to hyperreactivity. Our data also suggest enhanced force transmission associated with enrichment of structural proteins in the whole airway. These findings may lead to novel directions for treatment development in asthma.Intrapulmonary, but not tracheal, airway smooth muscle is hyperreactive in asthma, together with pro-contractile changes in the airway smooth muscle proteome. Several proteins were identified that could be targeted for treatment of the hyperreactivity. http://bit.ly/33esYSU ER -