TY -的T1 -嗜酸性粒细胞,嗜碱性粒细胞和2型mmune microenvironments in COPD-affected lung tissue JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.00110-2019 VL - 55 IS - 5 SP - 1900110 AU - Jogdand, Prajakta AU - Siddhuraj, Premkumar AU - Mori, Michiko AU - Sanden, Caroline AU - Jönsson, Jimmie AU - Walls, Andrew F. AU - Kearley, Jennifer AU - Humbles, Alison A. AU - Kolbeck, Roland AU - Bjermer, Leif AU - Newbold, Paul AU - Erjefält, Jonas S. Y1 - 2020/05/01 UR - //www.qdcxjkg.com/content/55/5/1900110.abstract N2 - Although elevated blood or sputum eosinophils are present in many patients with COPD, uncertainties remain regarding the anatomical distribution pattern of lung-infiltrating eosinophils. Basophils have remained virtually unexplored in COPD. This study mapped tissue-infiltrating eosinophils, basophils and eosinophil-promoting immune mechanisms in COPD-affected lungs.Surgical lung tissue and biopsies from major anatomical compartments were obtained from COPD patients with severity grades Global Initiative for Chronic Obstructive Lung Disease stages I–IV; never-smokers/smokers served as controls. Automated immunohistochemistry and in situ hybridisation identified immune cells, the type 2 immunity marker GATA3 and eotaxins (CCL11, CCL24).Eosinophils and basophils were present in all anatomical compartments of COPD-affected lungs and increased significantly in very severe COPD. The eosinophilia was strikingly patchy, and focal eosinophil-rich microenvironments were spatially linked with GATA3+ cells, including type 2 helper T-cell lymphocytes and type 2 innate lymphoid cells. A similarly localised and interleukin-33/ST2-dependent eosinophilia was demonstrated in influenza-infected mice. Both mice and patients displayed spatially confined eotaxin signatures with CCL11+ fibroblasts and CCL24+ macrophages.In addition to identifying tissue basophilia as a novel feature of advanced COPD, the identification of spatially confined eosinophil-rich type 2 microenvironments represents a novel type of heterogeneity in the immunopathology of COPD that is likely to have implications for personalised treatment.Highly localised Th2- and eosinophil-rich pockets were identified in COPD-affected lungs, which increased in number with increasing disease severity and included basophils. This exemplifies a novel type of heterogeneity in the immunopathology of COPD. http://bit.ly/2HexTco ER -