RT期刊文章SR电子T1通气早产儿肠肺轴不相似和下气道生态失调JF欧洲呼吸杂志JO Eur Respir J FD欧洲呼吸学会SP 1901909 DO 10.1183/13993003.01909-2019 VO 55 IS 5 A1 Gallacher, David A1 Mitchell, Emma A1 Alber, Dagmar A1 Wach, Richard188bet官网地址 A1 Klein, Nigel A1 Marchesi, Julian R. A1 Kotecha，Sailesh YR 2020 UL //www.qdcxjkg.com/content/55/5/1901909.abstract AB背景慢性早产儿肺病(CLD)，也称为支气管肺发育不良，是早产的一个主要后果，但微生物组在其发展中的作用尚不清楚。因此，我们评估了通气早产儿上、下气道细菌群落随时间的发展，并通过比较上、下气道细菌群落与粪便结果来评估肠肺轴。最后，我们评估了细菌群落是否与肺部炎症相关，以提示生态失调。方法对通气早产儿的上呼吸道(鼻咽吸液)、下呼吸道(气管吸液和支气管肺泡灌洗液)、肠道(粪便)等多个解剖部位进行连续取样。检测所有样品中的细菌DNA载量，并使用16S rRNA基因的V3-V4区进行测序。结果55例早产儿1102份(鼻咽引液539份，气管引液276份，支气管肺泡灌洗液89份，粪便198份)样本中，352份(32%)扩增出适合16S RNA基因测序。细菌负荷在出生时较低，随着时间的推移迅速增加，但在所有样本类型中都与主要的操作分类单位(OTUs)有关。上、下气道和肠道之间的细菌群落存在差异，婴儿下气道中发生了单独的非生物性炎症过程。个体OTUs与炎症标志物增加相关。Conclusions Taken together, these findings suggest that targeted treatment of the predominant organisms, including those not routinely treated, such as Ureaplasma spp., may decrease the development of CLD in preterm-born infants.Respiratory colonisation was acquired after birth and associated with a pro-inflammatory response, suggesting an infectious process was present in babies at risk of developing chronic lung disease of prematurity (CLD), thus providing a target to reduce CLD http://bit.ly/31C27iX