@文章{Gallacher1901909，作者= {Gallacher, David和Mitchell, Emma和Alber, Dagmar和Wach, Richard和Klein, Nigel和Marchesi, Julian R.和Kotecha, Sailesh}，标题={肠{\textendash}肺轴的不相似和通气早产儿下气道的生态失调}，体积={55}，编号={5}，位置-id ={1901909}，年份= {2020}，doi ={10.1183/13993003.01909-2019}，出版商={欧洲呼吸学会}，188bet官网地址慢性早产儿肺病(CLD)，也称为支气管肺发育不良，是早产的一个主要后果，但微生物组在其发展中的作用尚不清楚。因此，我们评估了通气早产儿上、下气道细菌群落随时间的发展，并通过比较上、下气道细菌群落与粪便结果来评估肠道肺轴。最后，我们评估了细菌群落是否与肺部炎症相关，以提示生态失调。方法对通气早产儿的上呼吸道(鼻咽吸液)、下呼吸道(气管吸液和支气管肺泡灌洗液)、肠道(粪便)等多个解剖部位进行连续取样。测量所有样品中的细菌DNA载量，并使用16S rRNA基因的V3{\textendash}V4区域进行测序。结果55例早产儿1102份(539份鼻咽引液，276份气管引液，89份支气管肺泡灌洗液，198份粪便)样本中，352份(32\%)扩增出适合16S RNA基因测序。细菌负荷在出生时较低，随着时间的推移迅速增加，但在所有样本类型中都与主要的操作分类单位(OTUs)有关。上、下气道和肠道之间的细菌群落存在差异，婴儿下气道中发生了单独的非生物性炎症过程。个体OTUs与炎症标志物增加相关。Conclusions Taken together, these findings suggest that targeted treatment of the predominant organisms, including those not routinely treated, such as Ureaplasma spp., may decrease the development of CLD in preterm-born infants.Respiratory colonisation was acquired after birth and associated with a pro-inflammatory response, suggesting an infectious process was present in babies at risk of developing chronic lung disease of prematurity (CLD), thus providing a target to reduce CLD http://bit.ly/31C27iX}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/55/5/1901909}, eprint = {//www.qdcxjkg.com/content/55/5/1901909.full.pdf}, journal = {European Respiratory Journal} }