TY -的T1 -“检测SV40病毒DNAnd viral antigens in malignant pleural mesothelioma.” M. Ramael, J. Nagels, H. Heylen, S. De Schepper, J. Paulussen, M. De Maeyer and C. Van Haesendonck. Eur Respir J 1999; 14: 1381–1386. JF - European Respiratory Journal JO - Eur Respir J DO - 10.1183/13993003.51381-1999 VL - 55 IS - 5 SP - 9951381 A2 - , Y1 - 2020/05/01 UR - //www.qdcxjkg.com/content/55/5/9951381.abstract N2 - From the authors:We reported in a previous paper on the possible association of simian vacuolating virus 40 (SV40)-like DNA and protein in malignant mesothelioma using the primed in situ labelling (PRINS) method with SVrev and SV2for, as well as a general polyomavirus primer PYVrev and immunohistochemistry using the antibody PAb280 [1]. As some concerns were raised regarding the specificity of the methods used, we felt it necessary to provide an explanatory clarifying note, including some new figures.We used in the original experiments several SV40 specific primers, including SVrev and SV2for, as well as a general polyomavirus primer PYVrev, in order to rule out the potential pitfall of aspecific binding of an oligonucleotide DNA primer (figure 1). PRINS signals were only observed in neoplastic mesothelioma cells. No PRINS signals were observed in stromal cells [1]. Positive control reaction is shown in a malignant epithelial mesothelioma with pan-chromosome primer in PRINS reaction (figure 1, insert). FIGURE 1 Most nuclei of neoplastic mesothelial cells display nuclear staining for simian vacuolating virus 40 (SV40) DNA with the primed in situ labelling (PRINS) method in an epithelial mesothelioma (SV2 for primer). … ER -