％0期刊文章％A McMahon，Derek B.％A Carey，Ryan M.％A Kohanski，Michael A.％A Tong，Charles C.L.％a papagiannopoulos，Peter％A adappa，Nithin D.％A Palmer，James N.％lee，Robert J.％T神经肽调节分泌和炎症在人类气道浆液中的分泌和炎症。％j欧洲呼吸杂志％p 1901386％v 55％n 4％x气道粘膜粘膜浆细胞是囊性纤维化跨膜电导调节剂（CFTR）表达的位点，对于导电气道中的流体分泌很重要。为了阐明神经肽如何调节浆液细胞，我们测试了人鼻涡流浆液性细胞分泌碳酸氢盐（HCO3-），对粘液聚合和抗菌肽功能很重要，并在cAMP-CAMP-EXTRACK-EXER-EXERVATINE CAMP-EXERVATINE CAMPARCACTIVE肠道肽（VIP）（VIP）和If cftrttr中刺激。用VIP刺激的浆液细胞表现出约15–20％的CAMP依赖性细胞体积降低，细胞内pH（PHI）的降低约为0.15单位，分别反映了Cl-和HCO3-分泌的激活。HCO3-分泌直接取决于CFTR，CF患者的细胞中不存在。相反，神经肽Y（NPY）减少了VIP诱发的CAMP增加，CFTR激活和Cl-/HCO3-分泌。在维持浆液表型的模型中，原代细胞的培养证实了VIP和NPY对液体和HCO3-分泌的激活和抑制作用。此外，VIP增强了分泌物的抗菌肽分泌和分泌的抗菌功效，而NPY降低了抗菌功效。相反，NPY通过需要CFTR的机制降低了细胞因子释放，而VIP降低了细胞因子的释放。 As levels of VIP and NPY are up-regulated in diseases like allergy, asthma, and chronic rhinosinusitis, the balance of these two peptides in the airway may control mucus rheology and inflammatory responses in serous cells. Furthermore, the loss of CFTR conductance in serous cells may contribute to CF pathophysiology by increasing serous cells inflammatory responses in addition to directly impairing Cl− and HCO3− secretion.VIP and NPY are neuropeptides up-regulated in allergy and asthma, respectively, which inversely regulate CFTR-dependent secretion and inflammation in airway submucosal gland serous cells, and which secrete much of the fluid that lines conducting airways http://bit.ly/2FWNT29 %U //www.qdcxjkg.com/content/erj/55/4/1901386.full.pdf