TY-Jour T1 - 人类气道腹泻细胞分泌和炎症的神经肽调节JF - 欧洲呼吸期Jo - Eur Respir J Do - 10.1183 / 13993003.01386-2019 VL - 55 IS - 4 SP - 1901386 Au - McMahon，Derek B.Au - Carey，Ryan M. Au - Kohanski，Michael A. Au-Tong，Charles C.L.Au - Papagiannopoulos，Peter Au - Adappa，Nithin D. Au - Palmer，James N. Au - Lee，Robert J. Y1 - 2020/04/01 UR - //www.qdcxjkg.com/content/55/4/1901386.Abstract N2 - 气道粘膜粘膜浆液细胞是囊性纤维化跨膜电导调节剂（CFTR）的表达位点，对导通气道的流体分泌很重要。为了阐明神经肽如何调节浆液细胞，我们测试了人鼻鼻甲浆液细胞分泌碳酸氢碳酸酯（HCO3-），对于用CAMP升高的血管活性肠肽（VIP）的刺激期间，对于粘液聚合和抗微生物肽功能而言是重要的，如果这需要CFTR，则在刺激升高和抗微生物肽功能期间。用VIP刺激刺激的浆液细胞表现出尤其依赖性降低细胞体积和细胞内pH（PHI）的〜0.15单位减少，分别反映了Cl-和HCO3分泌的活化。HCO3分泌直接依赖于CFTR，并且在CF患者中不存在于细胞中。相反，神经肽Y（npy）降低的vip诱发阵营增加，CFTR活化和CL- / HCO3分泌。在维持浆液表型的模型中初级浆液细胞的培养证实了VIP和NPY的活化和抑制效果，分别对流体和HCO3分泌。此外，VIP增强了分泌物的抗微生物肽分泌和分泌物的抗微生物效果，而NPY降低了抗菌药物。 In contrast, NPY enhanced cytokine release while VIP reduced cytokine release through a mechanism requiring CFTR. As levels of VIP and NPY are up-regulated in diseases like allergy, asthma, and chronic rhinosinusitis, the balance of these two peptides in the airway may control mucus rheology and inflammatory responses in serous cells. Furthermore, the loss of CFTR conductance in serous cells may contribute to CF pathophysiology by increasing serous cells inflammatory responses in addition to directly impairing Cl− and HCO3− secretion.VIP and NPY are neuropeptides up-regulated in allergy and asthma, respectively, which inversely regulate CFTR-dependent secretion and inflammation in airway submucosal gland serous cells, and which secrete much of the fluid that lines conducting airways http://bit.ly/2FWNT29 ER -