PT - JOURNAL ARTICLE AU - Ijpma, Gijs AU - Kachmar, Linda AU - Panariti, Alice AU - Matusovsky, Oleg S. AU - Torgerson, Dara AU - Benedetti, Andrea AU - Lauzon, Anne-Marie TI - Intrapulmonary airway smooth muscle ishyperreactive with a distinct proteome in asthma AID - 10.1183/13993003.02178-2019 DP - 2020 Jan 01 TA - European Respiratory Journal PG - 1902178 4099 - //www.qdcxjkg.com/content/early/2020/03/12/13993003.02178-2019.Short 4100 -http：//www.qdcxjkg.com/content/early/2020/03/03/12/13993003.02178-2019.Full AB-在哮喘发作期间气道的收缩是气道平滑肌（ASM）的结果。尽管普遍认为ASM在哮喘中是超额收缩，但尚未明确证明这一点。气道高反应性是否是单独增加ASM质量或每单位肌肉的收缩力增加的结果，直接决定了潜在的治疗途径。为了评估ASM是否是超额合同，我们对从肺内气管和气管中隔离的ASM进行了一系列机械测量来自人类肺。我们分析了ASM和整个气道蛋白质组，以验证蛋白质组学的转移是否有助于ASM性质的变化。我们报告说，分离的ASM收缩应激和特异性人类肺内支气管的僵硬增加，这是哮喘中抗气道耐药性增加的部位。其他收缩参数没有改变。无偏质谱数据的主成分分析显示，相对于ASM特异性蛋白，哮喘受试者的明确聚类。整个气道蛋白质组显示结构蛋白的上调。我们没有发现任何证据表明哮喘ASM中肌球蛋白活性的调节有所不同，我们表明ASM确实在哮喘内肺内气道的水平上具有过度反应性。 We identified several proteins that are upregulated in asthma that could contribute to hyperreactivity. Our data also suggest enhanced force transmission associated with enrichment of structural proteins in the whole airway. These findings may lead to novel directions for treatment development in asthma.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. IJpma has nothing to disclose.Conflict of interest: Dr. Kachmar has nothing to disclose.Conflict of interest: Dr. PANARITI has nothing to disclose.Conflict of interest: Dr. Matusovsky has nothing to disclose.Conflict of interest: Dr. Torgerson has nothing to disclose.Conflict of interest: Dr. Benedetti has nothing to disclose.Conflict of interest: Dr. Lauzon has nothing to disclose.