TY - JOUR T1 -哮喘肺内气道平滑肌高反应，具有独特的蛋白质组JF -欧洲呼吸杂志JO - Eur Respir J DO - 10.1183/13993003.02178-2019 SP - 1902178 AU - Ijpma, Gijs AU - Kachmar, Linda AU - Panariti, Alice AU - Matusovsky, Oleg S. AU - toorgerson, Dara AU - Benedetti, Andrea AU - Lauzon，Anne-Marie Y1 - 2020/01/01 UR - //www.qdcxjkg.com/content/early/2020/03/12/13993003.02178-2019.abstract N2 -哮喘加重期气道收缩是气道平滑肌(ASM)收缩的结果。虽然人们普遍认为哮喘的ASM是过度收缩的，但这并没有得到明确的证明。气道高反应性是单纯增加ASM质量的结果还是单位肌肉产生的收缩力的增加直接决定了治疗的潜在途径。为了评估ASM是否具有高收缩性，我们对从肺内气道和气管分离的ASM进行了一系列力学测量。我们分析了ASM和全气道蛋白质组，以验证蛋白质组的变化是否有助于ASM性质的变化。我们报告了哮喘患者肺内支气管(哮喘患者气道阻力增加的部位)特异的孤立ASM收缩应力和僵硬度的增加。其他收缩参数没有改变。无偏质谱数据的主成分分析显示哮喘受试者在ASM特异性蛋白方面有明显的聚类。全气道蛋白质组结构蛋白表达上调。我们没有发现任何证据表明哮喘性ASM中肌凝蛋白活性的调节存在差异。In conclusion, we showed that ASM is indeed hyperreactive at the level of intrapulmonary airways in asthma. We identified several proteins that are upregulated in asthma that could contribute to hyperreactivity. Our data also suggest enhanced force transmission associated with enrichment of structural proteins in the whole airway. These findings may lead to novel directions for treatment development in asthma.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. IJpma has nothing to disclose.Conflict of interest: Dr. Kachmar has nothing to disclose.Conflict of interest: Dr. PANARITI has nothing to disclose.Conflict of interest: Dr. Matusovsky has nothing to disclose.Conflict of interest: Dr. Torgerson has nothing to disclose.Conflict of interest: Dr. Benedetti has nothing to disclose.Conflict of interest: Dr. Lauzon has nothing to disclose. ER -