TY - JOUR T1 -人体离体肺灌注中肺修复的潜在治疗靶点JF -欧洲呼吸杂志JO - Eur Respir J DO - 10.1183/13993003.02222-2019 SP - 1902222 AU - Wong, Aaron AU - Zamel, Ricardo AU - Yeung, Jonathan AU - Bader, Gary D. AU - Dos Santos, Claudia C. AU - Bai, Xiaohui AU - Wang, Yubo AU - Keshavjee, Shaf AU - Liu，体外肺灌注(EVLP)技术已被开发用于评估边缘供肺的功能，这大大提高了供肺的利用率。EVLP也被探索作为供体肺修复的平台，通过损伤特异性治疗，如抗生素或纤维蛋白溶解剂。我们假设，移植和EVLP之间共享的积极表达通路可能揭示了移植前肺修复的共同损伤机制和潜在的治疗靶点。材料和方法对“脑死亡后捐赠”肺的外周组织活检进行了回顾性转录组分析，其中有46对移植前/移植后对和49对evlp前/移植后对。途径分析用于识别和比较供体肺对移植和EVLP的反应。结果22条通路在移植过程中主要富集，包括淋巴细胞活化和细胞死亡的上调，代谢的下调。EVLP主要富集了8条通路，包括白细胞功能下调和血管突起上调。27种途径普遍富集，包括先天性炎症激活、细胞死亡、热应激以及代谢和蛋白质合成的下调。在炎症簇中，TLR/MYD88信号具有最多的节点数，是炎症的中心。 These mechanisms have been previously speculated as major mechanisms of acute lung injury in animal models.Conclusion EVLP and transplantation share common molecular features of injury including innate inflammation and cell death. Blocking these pathways during EVLP may allow for lung repair prior to transplantation.FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. Wong has nothing to disclose.Conflict of interest: Dr. Zamel has nothing to disclose.Conflict of interest: Dr. Yeung has nothing to disclose.Conflict of interest: Dr. Bader has nothing to disclose.Conflict of interest: Dr. Dos Santos has nothing to disclose.Conflict of interest: Dr. Bai has nothing to disclose.Conflict of interest: Dr. Wang has nothing to disclose.Conflict of interest: Dr. Keshavjee has nothing to disclose.Conflict of interest: Dr. Liu has nothing to disclose. ER -