TY -的T1 -微分DNA甲基化在持续哮喘和哮喘缓解期支气管活检JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.01280 -2019欧元六世- 55 - 2 SP - 1901280 AU Vermeulen Cornelis J . AU -徐,陈健盟——Vonk Judith m . AU - 10 Hacken,尼克•高韧性非盟-华Wim盟——Heijink艾琳h . AU - Nawijn卡坦c . AU - Boekhoudt Jeunard AU - van Oosterhout Antoon说道J . AU -阿弗莱克,凯伦盟——WeckmannMarkus AU - Koppelman, Gerard H. AU - van den Berge, Maarten Y1 - 2020/02/01 UR - //www.qdcxjkg.com/content/55/2/1901280.abstract N2 -约40%的哮喘患者哮喘症状得到缓解。更好地理解导致哮喘缓解的生物学途径可能为哮喘的治疗提供新的靶点。作为基因调控的重要机制之一，DNA甲基化的研究为基因调控提供了一条新的途径。我们的目的是鉴别哮喘缓解组与哮喘持续组或健康对照组支气管活检中表观基因组DNA甲基化水平的差异。我们使用limma软件包分析了26例持续性哮喘患者、39例缓解期患者和70例健康对照组支气管活检的差异DNA甲基化。利用梳状-p工具鉴别差异甲基化区域。cpg位点的DNA甲基化与来自同一活检的邻近基因的表达有关，以了解其功能。4个cpg位点和42个区域在持续性哮喘和缓解之间存在差异甲基化。两个位点的DNA甲基化与ACKR2和DGKQ的基因表达有关。在缓解组和健康对照组之间，1163个cpg位点和328个区域被差异甲基化。DNA甲基化与一组基因在纤毛上皮的表达有关。CpGs differentially methylated between remission and persistent asthma identify genetic loci associated with resolution of inflammation and airway responsiveness. Despite the absence of symptoms, remission subjects have a DNA methylation profile that is distinct from that of healthy controls, partly due to changes in cellular composition, with a higher gene expression signal related to ciliated epithelium in remission versus healthy controls.Former asthma patients have epigenetic modifications not present in current asthma which are associated with the activity of genes involved in the resolution of inflammation. Their epigenetic profile also shows them to be different from healthy controls. http://bit.ly/2BGmCPl ER -