％0期刊论文％A Vermeulen的，科内利斯J.％A旭，成建％A Vonk，作者Judith M.％一个10克勤，尼克H.T.％A Timens，维姆％A Heijink，艾琳H.％A Nawijn，马亭C.％A Boekhoudt，Jeunard％A面包车Oosterhout的，安东J.％A阿弗莱克，克伦％A Weckmann，马库斯％A的Koppelman，杰拉德H.％甲范登Berge的，马腾％T微分DNA甲基化在缓解％d 2020％R 10.1183 / 13993003.01280-2019％Ĵ欧洲呼吸杂志％P 1901280％V 55％N 2％X大约40％的持续性哮喘和哮喘之间支气管活检哮喘患者的体验哮喘症状缓解。更好地理解生物途径导致哮喘缓解期可提供洞察哮喘新的治疗靶点。由于基因调控的一个重要机制，DNA甲基化研究提供了一个有前途的方法。我们的目的是确定在哮喘缓解受试者和持续性哮喘或健康controls.We之间支气管活检表观宽DNA甲基化水平的差异分析支气管活检差动DNA甲基化从26名受试者持续性哮喘，39名缓解受试者和70例健康控制，使用LIMMA包。梳 - 对工具用于鉴定差异甲基化区域。CpG的位点的DNA甲基化相关联，以从相同的活检附近的基因的表达来了解function.Four CpG的位点和持续性哮喘和缓解之间被差异甲基化的42个区域。 DNA methylation at two sites was correlated in cis with gene expression at ACKR2 and DGKQ. Between remission subjects and healthy controls 1163 CpG-sites and 328 regions were differentially methylated. DNA methylation was associated with expression of a set of genes expressed in ciliated epithelium.CpGs differentially methylated between remission and persistent asthma identify genetic loci associated with resolution of inflammation and airway responsiveness. Despite the absence of symptoms, remission subjects have a DNA methylation profile that is distinct from that of healthy controls, partly due to changes in cellular composition, with a higher gene expression signal related to ciliated epithelium in remission versus healthy controls.Former asthma patients have epigenetic modifications not present in current asthma which are associated with the activity of genes involved in the resolution of inflammation. Their epigenetic profile also shows them to be different from healthy controls. http://bit.ly/2BGmCPl %U //www.qdcxjkg.com/content/erj/55/2/1901280.full.pdf