TY - T1的过量粘液粘度和气道脱水影响COPD气道间隙JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.00419 -2019欧元六世- 55 - 1 SP - 1900419 AU -林,维维安y盟——Kaza Niroop盟——Birket Susan e . AU -金,哈里森AU -爱德华兹,劳埃德·J . AU -拉方丹则詹妮弗盟——刘Linbo盟——Mazur滨AU - Byzek,斯蒂芬·a . AU -哈贾斯汀盟——Tearney Guillermo j . AU -拉美国Vamsee AU -罗,Steven m . Y1 - 2020/01/01 UR - //www.qdcxjkg.com/content/55/1/1900419.abstract N2 -吸烟会损害呼吸道粘液清除的机制并不清楚。最近，我们建立了一种表现为慢性支气管炎的卷烟诱发的慢性阻塞性肺疾病(COPD)雪貂模型。我们研究了吸烟对粘液纤毛运输(MCT)的影响。成年雪貂暴露于香烟烟雾中6个月，用锝标记DTPA滞留法测定体内黏液纤毛清除率。切除气管用微光学相干断层摄影成像。采用组织学和微粒追踪微流变学方法评估人气道上皮细胞和雪貂体外气道黏液的变化。重复测量的线性混合模型确定了MCT的关键决定因素。与空气对照相比，暴露在香烟烟雾中的雪貂表现出粘液分泌过多、黏液纤毛清除延迟(−89.0%，p<0.01)和气管MCT受损(−29.4%，p<0.05)。胆碱能刺激增加气道表面液体(ASL)深度(5.8±0.3 ~ 7.3±0.6µm, p<0.0001)和恢复MCT(6.8±0.8 ~ 12.9±1.2 mm·min - 1, p<0.0001)。 Mixed model analysis controlling for covariates indicated smoking exposure, mucus hydration (ASL) and ciliary beat frequency were important predictors of MCT. Ferret mucus was hyperviscous following smoke exposure in vivo or in vitro, and contributed to diminished MCT. Primary cells from smokers with and without COPD recapitulated these findings, which persisted despite the absence of continued smoke exposure.Cigarette smoke impairs MCT by inducing airway dehydration and increased mucus viscosity, and can be partially abrogated by cholinergic secretion of fluid secretion. These data elucidate the detrimental effects of cigarette smoke exposure on mucus clearance and suggest additional avenues for therapeutic intervention.Mucus abnormalities impact COPD airway clearance http://bit.ly/2VEhFjd ER -