RT期刊文章SR电子T1循环等离子体在阻塞性睡眠呼吸暂停和反向浸渍液血压摩根富林明欧洲呼吸杂志乔和J FD欧元欧洲呼吸学会SP 1901072 10.1183/13993003.01072 -2019签证官55 1 A1 Khalyfa,大卫·陈A1 Abdelnaby A1格扎尔Wen-Ching A1安德拉德,豪尔赫A1普拉萨德,188bet官网地址摘要阻塞性睡眠呼吸暂停(OSA)可增加24小时血压不下降的风险，而24小时血压不下降是心血管疾病(CVD)的一个独立危险因素。我们检测了未治疗的OSA患者正常蘸压(NDBP)和反向蘸压(RDBP)(一种不蘸压的极端形式)的外体microRNA (miRNA)的差异表达，以了解OSA患者不蘸压的机制。方法46例(15 RDBP对31 NDBP)符合OSA严重程度(呼吸事件指数32.6±22.5对32.2±18.1事件·h−1;年龄(54.8±12.9岁与49±9.9岁);体重指数(36.2±6.6)与34.4±6.8 kg·m−2;包括p = 0.4)。通过流式细胞术对血浆外泌体进行定性，并对体外培养的内皮细胞进行功能报告试验。微阵列分析了外泌体miRNA的表达，并进行了生物信息学分析。结果RDBP患者的外泌体增加了内皮细胞紧密连接的通透性和粘附分子的表达。主成分分析显示，两组miRNA序列数据均有严格的分离和鉴定。 A restricted and validated signature of exosomal miRNAs was identified in the RDBP versus NDBP group. Their predicted target genes involved phosphatidylinositol 3-kinase-Akt (p=0.004), Ras (p=3.42E-05), Wnt (p=0.003) and hypoxia inducible factor-1 signalling (p=0.04), inflammatory mediator regulation of transient receptor potential channels (p=0.01), and several cancer-related pathways.Conclusions Patients with RDBP have altered miRNA cargoes in circulating exosomes that invoke in vitro endothelial dysfunction. A selected number of circulating exosomal miRNAs play an important role in abnormal circadian regulation of blood pressure and may provide prognostic biomarkers of CVD risk in OSA.A selected number of circulating exosomal miRNAs play an important role in abnormal circadian regulation of blood pressure and may provide prognostic biomarkers of cardiovascular risk in OSA http://bit.ly/2VPRFl4