PT -期刊文章盟Danopoulos Soula AU - Bhattacharya Soumyaroop AU -马里安尼,Thomas j . AU -艾尔阿拉姆,丹尼斯TI -转录描述的人类肺细胞识别小说间叶细胞谱系标记援助- 10.1183/13993003.00746 -2019 DP - 2020年1月01 TA -欧洲呼吸杂志》第六PG - 1900746 - 55 IP - 1 4099 - //www.qdcxjkg.com/content/55/1/1900746.short 4100 - //www.qdcxjkg.com/content/55/1/1900746.full所以欧元和J2020 1月01;肺间充质在成熟的呼吸系统中产生多种不同的细胞谱系，包括气道和血管的平滑肌细胞。然而，对人类肺的规格和间充质细胞多样性缺乏全面的了解。方法对胎儿肺组织进行单细胞RNA测序分析。在Seurat中进行典型相关分析、聚类、聚类标记基因鉴定和t分布随机近邻嵌入表示。细胞群体使用ToppFun进行注释。免疫组化和原位杂交技术用于验证关键标记基因的时空表达模式。结果:我们鉴定出分子上不同的群体，它们代表了“承诺的”胎儿人肺内皮细胞、周细胞和平滑肌细胞。早期内皮细胞系表达“经典”内皮细胞标志物(血小板内皮细胞粘附分子/CD31和claudin 5)，而周细胞表达血小板来源的生长因子受体-β， Thy-1膜糖蛋白和基底膜分子(IV型胶原蛋白，层粘连蛋白和蛋白聚糖)。我们观察到一大群“非特异性”人肺间充质前细胞，其特征是I型胶原蛋白和多种弹性蛋白纤维基因(ELN, MFAP2和FBN1)的表达。我们通过α2-平滑肌肌动蛋白(ACTA2)的表达密切地描述了间充质谱系的多样性。 Two cell populations, with the highest levels of ACTA2 transcriptional activity, expressed unique sets of markers associated with airway or vascular smooth muscle cells. Spatiotemporal analysis of these marker genes confirmed early and persistent spatial specification of airway (HHIP, MYLK and IGF1) and vascular (NTRK3 and MEF2C) smooth muscle cells in the developing human lung.Conclusion Our data suggest that specification of distinct airway and vascular smooth muscle cell phenotypes is established early in development and can be identified using the markers we provide.Data presented here provide novel information on molecular markers for multiple cell types within the fetal human lung. In particular, we identify new, putative markers capable of spatially distinguishing airway and vascular smooth muscle cells. http://bit.ly/2mjgOax