TY -的T1 -肺动脉内皮细胞的文化从肺动脉导管气球提示:考虑用于肺血管疾病JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.01313 -2019欧元SP - 1901313 AU -科里大肠Ventetuolo AU -杰森·m·Aliotta AU -朱莉Braza盟Havovi Chichger AU -马克内尔AU -唐纳德McGuirl AU -克里斯托弗·J·穆林AU -朱莉牛顿盟曼迪佩雷拉Peter J . Quesenberry AU -艾米Princiotto盟盟-托马斯·沃尔什AU -玛丽Whittenhall AU -詹姆斯·r·科林格盟-伊丽莎白·o·哈林顿Y1 - 2020/01/01 UR - //www.qdcxjkg.com/content/early/2019/12/12/13993003.01313 - 2019. -抽象N2 -内皮功能障碍是肺动脉高血压的标志(PAH)但没有建立方法研究肺动脉内皮细胞(PAECs)从生活的病人。我们寻求文化PAECs肺动脉导管(PAC)气球中使用正确的心脏catheterisation (RHC),描述成功的文化,试图描述PAEC行为。PAECs主要生长在文化融合和内皮细胞表型确认。标准检测细胞凋亡、迁移和管形成通道3 - 8之间进行。我们收集49 PAC技巧来自45个科目与成功PAEC文化从19(39%)气球。没有主题的人口差异或RHC程序细节成功与失败。有更高的成功但无意义的比例(10/19,53%)和不成功(9/30,30%)尝试从受试者符合血流动力学标准多环芳烃(p = 0.10)。成功的文化更可能在较低的受试者心脏指数(p = 0.03)和更高的肺血管阻力(p = 0.04)。PAECs从主题与特发性肺动脉高压抗凋亡相比商业PAECs (p = 0.04),减少迁移PAECs相比,从一个主题portopulmonary高血压与高心输出量(p = 0.01)。PAECs与艾滋病毒相关主题多环芳烃形成少(p = 0.01)和短(p = 0.02)船网络商业PAECs相比。持续的文化和人物塑造的PAECs RHC气球是可行的,特别是在多环芳烃高血流动力学的负担。这种技术可以提供洞察PAH发病期间内皮功能障碍。FootnotesThis manuscript has recently been accepted for publication in the European Respiratory Journal. It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article.Conflict of interest: Dr. Ventetuolo reports grants from National Institutes of Health , during the conduct of the study; grants from United Therapeutics and Eiger to her institution, personal fees from Acceleron Pharma, outside the submitted work; and Spouse is an employee of CVS Health.Conflict of interest: Dr. Aliotta has nothing to disclose.Conflict of interest: Dr. Braza has nothing to disclose.Conflict of interest: Dr. Chichger has nothing to disclose.Conflict of interest: Dr. Dooner has nothing to disclose.Conflict of interest: Dr. McGuirl has nothing to disclose.Conflict of interest: Dr. Mullin has nothing to disclose.Conflict of interest: Dr. Newton has nothing to disclose.Conflict of interest: Dr. Pereira has nothing to disclose.Conflict of interest: Dr. Princiotto has nothing to disclose.Conflict of interest: Dr. Quesenberry has nothing to disclose.Conflict of interest: Dr. Walsh has nothing to disclose.Conflict of interest: Dr. Whittenhall has nothing to disclose.Conflict of interest: Dr. Klinger has nothing to disclose.Conflict of interest: Dr. Harrington has nothing to disclose. ER -